Lv Na, Ni Jinjing, Fang Shiqi, Liu Yue, Wan Shuang, Sun Chao, Li Jun, Zhou Aiping
Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
Department of Laboratory Medicine, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200123, China.
Microorganisms. 2024 Aug 13;12(8):1667. doi: 10.3390/microorganisms12081667.
species are causal pathogens instrumental in human food-borne diseases. The pandemic survey related to multidrug resistant (MDR) genomics enables the prevention and control of their dissemination. Currently, serotype Mbandaka is notorious as a multiple host-adapted non-typhoid . However, its epidemic and MDR properties are still obscure, especially its genetic determinants accounting for virulence and MD resistance. Here, we aim to characterize the genetic features of a strain SMEH pertaining to Mbandaka ( Mbandaka), isolated from the patient's hydropericardium, using cell infections, a mouse model, antibiotic susceptibility test and comparative genomics. The antibiotic susceptibility testing showed that it could tolerate four antibiotics, including chloramphenicol, tetracycline, fisiopen and doxycycline by Kirby-Bauer (K-B) testing interpreted according to the Clinical and Laboratory Standards Institute (CLSI). Both the reproducibility in RAW 264.7 macrophages and invasion ability to infect HeLa cells with strain SMEH were higher than those of Typhimurium strain 14028S. In contrast, its attenuated virulence was determined in the survival assay using a mouse model. As a result, the candidate genetic determinants responsible for antimicrobial resistance, colonization/adaptability and their transferability were comparatively investigated, such as bacterial secretion systems and pathogenicity islands (SPI-1, SPI-2 and SPI-6). Moreover, collective efforts were made to reveal a potential role of the plasmid architectures in Mbandaka as the genetic reservoir to transfer or accommodate drug-resistance genes. Our findings highlight the essentiality of antibiotic resistance and risk assessment in Mbandaka. In addition, genomic surveillance is an efficient method to detect pathogens and monitor drug resistance. The genetic determinants accounting for virulence and antimicrobial resistance underscore the increasing clinical challenge of emerging MDR Mbandaka isolates, and provide insights into their prevention and treatment.
某些物种是导致人类食源性疾病的病原体。与多重耐药（MDR）基因组学相关的大流行调查有助于预防和控制它们的传播。目前，Mbandaka血清型作为一种多宿主适应性非伤寒菌而声名狼藉。然而，其流行和MDR特性仍不清楚，尤其是其导致毒力和MDR抗性的遗传决定因素。在这里，我们旨在通过细胞感染、小鼠模型、抗生素敏感性测试和比较基因组学，对从患者心包积水分离出的一株与Mbandaka相关的菌株SMEH的遗传特征进行表征。抗生素敏感性测试表明，根据临床和实验室标准协会（CLSI）的解释，通过Kirby-Bauer（K-B）测试，它可以耐受四种抗生素，包括氯霉素、四环素、菲西潘和强力霉素。菌株SMEH在RAW 264.7巨噬细胞中的重现性和感染HeLa细胞的侵袭能力均高于鼠伤寒沙门氏菌菌株14028S。相比之下，在使用小鼠模型的存活试验中确定了其减弱的毒力。结果，对负责抗菌抗性、定植/适应性及其转移性的候选遗传决定因素进行了比较研究，例如细菌分泌系统和致病岛（SPI-1、SPI-2和SPI-6）。此外，还共同努力揭示Mbandaka中质粒结构作为转移或容纳耐药基因的遗传库的潜在作用。我们的研究结果强调了Mbandaka中抗生素抗性和风险评估的重要性。此外，基因组监测是检测病原体和监测耐药性的有效方法。导致毒力和抗菌抗性的遗传决定因素突出了新兴MDR Mbandaka分离株日益增加的临床挑战，并为其预防和治疗提供了见解。
