Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
Appl Microbiol Biotechnol. 2018 Nov;102(22):9517-9529. doi: 10.1007/s00253-018-9358-x. Epub 2018 Sep 14.
Corynebacterium glutamicum (C. glutamicum), an important industrial workhorse, is capable of efficiently producing a variety of value-added chemicals and fuels beyond amino acids. C. glutamicum has a broad natural substrate spectrum and can simultaneously utilize various carbon sources in blends. The substrate spectrum of C. glutamicum has been further extended by detailed knowledge of carbon core metabolism and well-established genetic tools and engineering strategies. At present, many pathways have been successfully engineered in C. glutamicum for access to alternative renewable sources to produce natural or non-natural products, making C. glutamicum a promising and favorable microbial cell factory. In this review, we mainly focus on synthetic biology and metabolic engineering strategies for developing synthetic strains that grow on renewable sources to produce the target products. At the same time, we also explore the promotion and future challenges of existing synthetic biology platforms for industrial platform microorganism metabolic engineering efforts.
谷氨酸棒杆菌（Corynebacterium glutamicum）是一种重要的工业生产工具，能够高效生产各种附加值化学品和燃料，不仅限于氨基酸。谷氨酸棒杆菌具有广泛的天然底物谱，可以同时混合利用各种碳源。通过对碳核心代谢的详细了解以及成熟的遗传工具和工程策略，进一步扩展了谷氨酸棒杆菌的底物谱。目前，已经在谷氨酸棒杆菌中成功构建了许多途径，以获取替代可再生资源来生产天然或非天然产品，使谷氨酸棒杆菌成为一种有前途和有利的微生物细胞工厂。在本文综述中，我们主要关注合成生物学和代谢工程策略，用于开发以可再生资源为生长基质并生产目标产物的合成菌株。同时，我们还探讨了现有合成生物学平台对工业平台微生物代谢工程努力的促进作用和未来挑战。
