Morales Princess, Tang Patrick, Mariano Elaine, Gopalan Arun, Aji Nisha, Pérez-López Andrés, Suleiman Mohammed
Department of Pathology, Sidra Medicine, Doha P.O. Box 26999, Qatar.
Department of Pathology and Laboratory Medicine, Weill Cornell Medicine in Qatar, Doha, P.O Box 24144, Qatar.
Microorganisms. 2024 Aug 18;12(8):1704. doi: 10.3390/microorganisms12081704.
Bloodstream infections (BSIs) are life-threatening infections for which a timely initiation of appropriate antimicrobial therapy is critical. Antibiotic susceptibility testing (AST) directly performed on positive blood culture broths can help initiate targeted antibiotic therapy sooner than the standard AST performed on colonies isolated on solid media after overnight incubation. Faster antimicrobial susceptibility testing (AST) results can improve clinical outcomes, and reduce broad-spectrum antimicrobial consumption and healthcare-associated costs in sepsis. In this study, we evaluated the accuracy of a direct AST inoculation method on the BD Phoenix M50 system using serum separator tubes to harvest bacteria from positive pediatric blood culture bottles. Direct AST was performed on 132 monomicrobial pediatric blood culture bottles that were positive for Enterobacterales (65; 49.2%), (46; 34.8%), and non-fermenting Gram-negative bacilli (21; 16%). Overall, the categorical and essential agreements between the direct method and standard method were 99.6% and 99.8%, respectively. Very major, major, and minor error rates were 0.1%, 0.09%, and 0.20% respectively. Direct AST performed on pediatric blood culture bottles using BD Phoenix M50 can quickly provide accurate susceptibility information to guide antimicrobial therapy in patients with BSI.
血流感染（BSIs）是危及生命的感染，及时开始适当的抗菌治疗至关重要。直接在阳性血培养肉汤上进行的抗生素敏感性试验（AST）比在过夜培养后在固体培养基上分离的菌落进行的标准AST能更快地启动针对性抗生素治疗。更快的抗菌药物敏感性试验（AST）结果可改善临床结局，并减少脓毒症中广谱抗菌药物的使用和医疗相关成本。在本研究中，我们评估了在BD Phoenix M50系统上使用血清分离管从阳性儿科血培养瓶中采集细菌的直接AST接种方法的准确性。对132个单一微生物的儿科血培养瓶进行了直接AST检测，这些血培养瓶中肠杆菌科细菌阳性（65个；49.2%）、 （46个；34.8%）和非发酵革兰氏阴性杆菌阳性（21个；16%）。总体而言，直接法与标准法之间的分类一致性和基本一致性分别为99.6%和99.8%。极重大、重大和微小错误率分别为0.1%、0.09%和0.20%。使用BD Phoenix M50对儿科血培养瓶进行直接AST检测可快速提供准确的药敏信息，以指导血流感染患者的抗菌治疗。
