GeneChem Inc., Daejeon 34025, Republic of Korea.
Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
Nutrients. 2024 Aug 7;16(16):2600. doi: 10.3390/nu16162600.
Sialyllactose (SL) is a functional human milk oligosaccharide essential for immune support, brain development, intestinal maturation, and antiviral defense. However, despite its established health benefits, the effect of SL on exercise performance and muscle mass in mice remains unknown. Here, we aimed to investigate, for the first time, the effects of 6'-SL on muscle functions. Seven-week-old male C57BL/6J mice were administered 100 mg/kg 6'-SL for 12 weeks, after which exhaustive treadmill performance was conducted. Moreover, muscle strength was examined by grip strength, and muscle phenotype characteristics such as muscle mass, muscle fiber size, and muscle protein expression were also examined. The administration of 6'-SL significantly improved exhaustive treadmill performance metrics, including distance and exhaustion time. Grip strength was also increased by 6'-SL administration. Additionally, 6'-SL increased muscle mass in both the gastrocnemius (GAS) and soleus. 6'-SL administration led to an increase in the minimum Feret's diameter and the protein expression of total myosin heavy chain in the GAS muscle. In conclusion, 6'-SL administration in vivo led to increased running distance and time by increasing muscle mass and strength. These findings collectively indicate that 6'-SL is a potential agent for improving muscle health and exercise performance.
唾液酸乳糖（SL）是一种功能性人乳寡糖，对免疫支持、大脑发育、肠道成熟和抗病毒防御至关重要。然而，尽管其具有已确立的健康益处，但 SL 对小鼠运动表现和肌肉质量的影响尚不清楚。在这里，我们首次旨在研究 6'-SL 对肌肉功能的影响。将 7 周龄雄性 C57BL/6J 小鼠给予 100mg/kg 6'-SL 持续 12 周，然后进行竭性跑步机性能测试。此外，通过握力检查肌肉力量，还检查了肌肉表型特征，如肌肉质量、肌纤维大小和肌肉蛋白表达。6'-SL 的给药显著改善了竭性跑步机性能指标，包括距离和疲劳时间。6'-SL 的给药还增加了握力。此外，6'-SL 增加了腓肠肌（GAS）和比目鱼肌的肌肉质量。6'-SL 给药导致 GAS 肌肉的最小 Feret 直径和总肌球蛋白重链的蛋白表达增加。总之，体内给予 6'-SL 通过增加肌肉质量和力量，导致跑步距离和时间增加。这些发现共同表明 6'-SL 是一种改善肌肉健康和运动表现的潜在药物。
