Li Qingyong, Yu Xin, Ye Lin, Hou Tongyu, Liu Yi, Liu Guiming, Wang Qing, Zhang Defeng
Fisheries Research and Extension Center of Huizhou, Huizhou 516055, China.
Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China.
Pathogens. 2024 Jul 31;13(8):647. doi: 10.3390/pathogens13080647.
Outbreaks of bacterial diseases occur in farmed Chinese longsnout catfish (). Due to limited information on aquatic variicola-infected animals, this study aimed to identify strain LL2208 isolated from diseased , determine its biological features, and evaluate its risk to public health. Strain LL2208 was tested for molecular identification, challenge, string, biofilm formation, and antimicrobial susceptibility. Furthermore, the whole genome of the strain was sequenced and analyzed. Based on molecular identification, strain LL2208 was identified as . Artificial infection showed that this strain was moderately virulent to with an LD = 7.92 × 10 CFU/mL. Antibiotic sensitivity tests showed that this strain was resistant to penicillins, macrolides, aminoglycosides, amphenicols, glycopeptides, and lincosamide, indicating multidrug resistance. Strain LL2208 has a genome size of 5,557,050 bp, with a GC content of 57.38%, harboring 30 antimicrobial resistance genes and numerous virulence-related genes. Its molecular type was ST595-KL16-O5. Collinearity analysis showed that strain LL2208 was highly similar to the human-derived strain. In conclusion, the multidrug-resistant and virulent strain LL2208 was isolated from fish and may have originated from humans. These results provide a foundation for further studies on the transmission of between humans and aquatic animals.
养殖的长吻鮠出现细菌性疾病暴发。由于关于感染水生变种的动物的信息有限，本研究旨在鉴定从患病长吻鮠分离出的LL2208菌株，确定其生物学特性，并评估其对公共卫生的风险。对LL2208菌株进行了分子鉴定、攻毒、菌绳形成、生物膜形成和药敏试验。此外，对该菌株的全基因组进行了测序和分析。基于分子鉴定，LL2208菌株被鉴定为[具体菌名未给出]。人工感染表明该菌株对长吻鮠具有中度毒力，半数致死剂量（LD）= 7.92×10 CFU/mL。药敏试验表明该菌株对青霉素类、大环内酯类、氨基糖苷类、氯霉素类、糖肽类和林可酰胺类耐药，表明其具有多重耐药性。LL2208菌株基因组大小为5,557,050 bp，GC含量为57.38%，含有30个抗菌抗性基因和众多毒力相关基因。其分子型为ST595-KL16-O5。共线性分析表明LL2208菌株与源自人类的[具体菌名未给出]菌株高度相似。总之，从鱼类中分离出的多重耐药且有毒力的[具体菌名未给出]菌株LL2208可能源自人类。这些结果为进一步研究[具体菌名未给出]在人和水生动物之间的传播提供了基础。
