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源自零售肉类的耐头孢菌素基因特征的系统发育学与转移

Phylogenetics and Mobilization of Genomic Traits of Cephalosporin-Resistant Originated from Retail Meat.

作者信息

Iwan Ewelina, Zając Magdalena, Bomba Arkadiusz, Olejnik Małgorzata, Skarżyńska Magdalena, Wasiński Bernard, Wieczorek Kinga, Tłuścik Katarzyna, Wasyl Dariusz

机构信息

Department of Omics Analyses, National Veterinary Research Institute, 57 Partyzantow, 24-100 Pulawy, Poland.

Department of Microbiology, National Veterinary Research Institute, 57 Partyzantow, 24-100 Pulawy, Poland.

出版信息

Pathogens. 2024 Aug 19;13(8):700. doi: 10.3390/pathogens13080700.

Abstract

Contaminations with cephalosporin-resistant across the food chain may pose a significant threat to public health because those antimicrobials are critically important in human medicine. The impact of the presented data is especially significant concerning Poland's role as one of the leading food producers in the EU. This work aimed to characterize the genomic contents of cephalosporin-resistant (n = 36) isolated from retail meat to expand the official AMR monitoring reported by EFSA. The ESBL mechanism was predominant (via and ), with the AmpC-type represented by the variant. The strains harbored multiple resistance genes, mainly conferring resistance to aminoglycosides, sulfonamides, trimethoprim, tetracyclines. In some isolates, virulence factors-including intimin () and its receptor () were detected, indicating significant pathogenic potential. Resistance genes showed a link with IncI1 and IncB/O/K/Z plasmids. Cephalosporinases were particularly linked to ISEc9/ISEc1 ( and ). The association of virulence with mobile elements was less common-mostly with IncF plasmids. The analysis of isolated from retail meat indicates accumulation of ARGs and their association with various mobile genetic elements, thus increasing the potential for the transmission of resistance across the food chain.

摘要

整个食物链中对头孢菌素耐药的污染可能对公众健康构成重大威胁,因为这些抗菌药物在人类医学中至关重要。所呈现数据的影响对于波兰作为欧盟主要食品生产国之一的角色而言尤为显著。这项工作旨在表征从零售肉类中分离出的对头孢菌素耐药的(n = 36)的基因组内容,以扩展欧洲食品安全局(EFSA)报告的官方抗菌药物耐药性监测。ESBL机制占主导地位(通过和),AmpC型由变体代表。这些菌株携带多种耐药基因,主要赋予对氨基糖苷类、磺胺类、甲氧苄啶、四环素类的耐药性。在一些分离株中,检测到毒力因子,包括紧密素()及其受体(),表明具有显著的致病潜力。耐药基因与IncI1和IncB/O/K/Z质粒有关联。头孢菌素酶尤其与ISEc9/ISEc1(和)有关。毒力与移动元件的关联较少见,主要与IncF质粒有关。对从零售肉类中分离出的的分析表明,抗菌药物耐药基因的积累及其与各种移动遗传元件的关联,从而增加了耐药性在食物链中传播的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f2b/11357031/596211211d83/pathogens-13-00700-g001a.jpg

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