García-Sobrino Rubén, Casado-Losada Isabel, Caltagirone Carmen, García-Crespo Ana, García Carolina, Rodríguez-Hernández Juan, Reinecke Helmut, Gallardo Alberto, Elvira Carlos, Martínez-Campos Enrique
Polymer Functionalization Group, Departamento de Química Macromolecular Aplicada, Instituto de Ciencia y Tecnología de Polímeros-Consejo Superior de Investigaciones Científicas (ICTP-CSIC) Calle Juan de la Cierva, n° 3, 28006 Madrid, Spain.
Group of Organic Synthesis and Bioevaluation, Instituto Pluridisciplinar, Universidad Complutense de Madrid (UCM), Associated Unit to the ICTP-IQM-CSIC, Paseo Juan XXIII, n° 1, 28040 Madrid, Spain.
Pharmaceutics. 2024 Aug 13;16(8):1063. doi: 10.3390/pharmaceutics16081063.
New clinical strategies for treating severe bone and cartilage injuries are required, especially for use in combination with implant procedures. For this purpose, p(VCL-co-HEMA) thermosensitive hydrogels have been activated with icariin-loaded nanoparticles to be used as bone-cell-harvesting platforms. Supercritical CO-SAS technology has been applied to encapsulate icariin, a small molecule that is involved in osteoblastic differentiation. Thus, physical-chemical analysis, including swelling and transmittance, showed the impact of HEMA groups in hydrogel composition. Moreover, icariin (ICA) release from p(VCL-co-HEMA) platforms, including pVCL@ICA nanoparticles, has been studied to evaluate their efficacy in relevant conditions. Finally, the thermosensitive hydrogels' cell compatibility, transplant efficiency, and bone differentiation capacity were tested. This study identifies the optimal formulations for icariin-activated hydrogels for both control and HEMA formulations. Using this technique, osteoblastic sheets that were rich in collagen type I were successfully transplanted and recultivated, maintaining an optimal extracellular matrix (ECM) composition. These findings suggest a new cell-sheet-based therapy for bone regeneration purposes using customized and NP-activated pVCL-based cell platforms.
需要新的治疗严重骨和软骨损伤的临床策略，尤其是用于与植入手术联合使用时。为此，已用负载淫羊藿苷的纳米颗粒激活聚（VCL-共-HEMA）热敏水凝胶，用作骨细胞采集平台。超临界CO-SAS技术已应用于包裹淫羊藿苷，一种参与成骨细胞分化的小分子。因此，包括溶胀和透光率在内的物理化学分析显示了HEMA基团在水凝胶组成中的影响。此外，还研究了淫羊藿苷（ICA）从聚（VCL-共-HEMA）平台（包括pVCL@ICA纳米颗粒）的释放情况，以评估它们在相关条件下的功效。最后，测试了热敏水凝胶的细胞相容性、移植效率和成骨分化能力。本研究确定了淫羊藿苷激活水凝胶在对照和HEMA配方中的最佳配方。使用该技术，富含I型胶原蛋白的成骨细胞片成功移植并重新培养，维持了最佳的细胞外基质（ECM）组成。这些发现表明，使用定制的基于NP激活的pVCL细胞平台，可用于骨再生目的的基于细胞片的新疗法。
