Du Mengxue, Wang Deying, Li Jingyu, Zhu Taotao, Lyu Peng, Li Gang, Ding Yi, Liu Xinxin, Men Qingmei, Li Xiaofei, Sun Yongwang, Meng Lingzhi, Guo Shangjing
School of Agricultural Science and Engineering, Liaocheng University, Liaocheng 252000, China.
School of Life Science, Qingdao Agricultural University, Qingdao 266000, China.
Plants (Basel). 2024 Aug 6;13(16):2181. doi: 10.3390/plants13162181.
Flowering in plants is pivotal for initiating and advancing reproductive processes, impacting regional adaptation and crop yield. Despite numerous cloned and identified flowering time genes, research in cotton remains sparse. This study identified as a key determinant of the flowering time in cotton, demonstrating that its heterologous expression in accelerated flowering under LD conditions compared to WT. Transgenic plants exhibited upregulated expression of the flowering inducers , , , and , alongside downregulated expression of the repressors , , and , correlating with the earlier flowering phenotype. showed a constitutive expression pattern, with elevated levels in the leaves, petals, and flower buds, and was notably higher in early-maturing cotton varieties. Subcellular localization assays confirmed GhSWEET42's presence on the cell membrane. Transcriptome analysis between WT and -overexpressing plants revealed 2393 differentially expressed genes (DEGs), spanning 221 biological processes, 93 molecular functions, and 37 cellular components according to Gene Ontology (GO) enrichment analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis categorized the DEGs into metabolism and environmental information processing. These findings enhance the understanding of 's function and provide a foundation for elucidating the molecular mechanisms governing flowering time regulation in cotton.
植物开花对于启动和推进生殖过程至关重要，影响区域适应性和作物产量。尽管已经克隆和鉴定了许多开花时间基因，但棉花方面的研究仍然很少。本研究确定了 作为棉花开花时间的关键决定因素，表明其在 中的异源表达与野生型相比在长日照条件下加速了开花。转基因植物表现出开花诱导因子 、 、 和 的表达上调，同时抑制因子 、 和 的表达下调，这与较早开花的表型相关。 呈现组成型表达模式，在叶片、花瓣和花芽中水平升高，并且在早熟棉花品种中明显更高。亚细胞定位分析证实了GhSWEET42存在于细胞膜上。野生型和过表达 植物之间的转录组分析揭示了2393个差异表达基因（DEG），根据基因本体（GO）富集分析，这些基因跨越221个生物学过程、93个分子功能和37个细胞成分。京都基因与基因组百科全书（KEGG）通路分析将这些DEG分类为代谢和环境信息处理。这些发现增强了对 功能的理解，并为阐明棉花开花时间调控的分子机制提供了基础。
