Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510640, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2022 May 12;23(10):5391. doi: 10.3390/ijms23105391.
The Sugars Will Eventually be Exported Transporters (SWEET) family is a class of sugar transporters that play key roles in phloem loading, seed filling, pollen development and the stress response in plants. Here, a total of 18 genes were identified in physic nut ( L.) and classified into four clades by phylogenetic analysis. These genes share similar gene structures, and alternative splicing of messenger RNAs was observed for five of the genes. Three (//) of the JcSWEETs were found to possess transport activity for hexose molecules in yeast. Real-time quantitative PCR analysis of in different tissues under normal growth conditions and abiotic stresses revealed that most are tissue-specifically expressed, and 12 responded to either drought or salinity. The gene responded to drought and salinity stress in leaves, and the protein it encodes is localized in both the plasma membrane and the vacuolar membrane. The overexpression of in modified the flowering time and saline tolerance levels but not the drought tolerance of the transgenic plants. Together, these results provide insights into the characteristics of genes in physic nut and could serve as a basis for cloning and further functional analysis of these genes.
糖将最终被输出转运蛋白（SWEET）家族是一类糖转运蛋白，在韧皮部装载、种子填充、花粉发育和植物的应激反应中起着关键作用。在这里，总共鉴定出 18 个基因在麻疯树（L.）中，并通过系统发育分析将其分为四个分支。这些基因具有相似的基因结构，其中 5 个基因的信使 RNA 发生选择性剪接。三个（//）JcSWEET 被发现具有在酵母中运输己糖分子的活性。在正常生长条件和非生物胁迫下不同组织中的实时定量 PCR 分析表明，大多数基因是组织特异性表达的，有 12 个基因对干旱或盐度有反应。基因在叶片中对干旱和盐胁迫有反应，其编码的蛋白定位于质膜和液泡膜上。在转基因植物中过表达 基因改变了开花时间和耐盐性水平，但不改变耐旱性。总之，这些结果为麻疯树中基因的特性提供了深入了解，并可为这些基因的克隆和进一步功能分析提供基础。
