Antioxidant Active Phytochemicals in Explained by Aquaporin Mechanisms.

The antioxidant action of terngymnoside C () and hydroxytyrosol-1-glucoside (), isolated for the first time from the flower buds of , as well as katsumadin (), obtained from the seedless fruits, was evaluated using ABTS and HO- models. In silico docking analysis of , , and determined their affinity forces to the aquaporin monomers of the modeled protein 3 (AQP3) and human protein 7 (AQP7) channels that regulate the HO cell transport. The ABTS antiradical capacity of these compounds showed IC values of 22.00 μM (), 47.64 μM (), and 73.93 μM (). The antioxidant assay showed that at 25 µM () and 50 µM ( and ), the cells were protected from HO-oxidative stress. These compounds, together with quercetin and vitamin C, were explored through the modeled AQP3 and human AQP7 by molecular docking analysis. To explain these results, an antioxidant mechanism for the isolated compounds was proposed through blocking HO passage mediated by aquaporin transport. On the other hand, , and were not cytotoxic in a panel of three cancer cell lines.