Chen Hu, Liang Xingxing, Yang Zhangqi
Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, Nanning 530002, China.
Key Laboratory of Central South Fast-Growing Timber Cultivation of Forestry Ministry of China, Masson Pine Engineering Research Center of the State Forestry Administration, Nanning 530002, China.
Plants (Basel). 2024 Aug 11;13(16):2229. doi: 10.3390/plants13162229.
L. is one of the most important conifer species in southern China and is the mainstay of the forest ecosystem and timber production, yet low temperatures limit its growth and geographical distribution. This study used 30-day-old seedlings from families of varying cold-tolerance to examine the morphological traits of needles and stems, chlorophyll fluorescence characteristics, protective enzymes, and changes in starch and lignin under different low-temperature stresses in an artificial climate chamber. The results showed that the seedlings of exhibited changes in phenotypic morphology and tissue structure under low-temperature stress. Physiological and biochemical indexes such as protective enzymes, osmoregulatory substances, starch, and lignin responded to low-temperature stress. The cold-tolerant family increased soluble sugars, starch grain, and lignin content as well as peroxidase activity, and decreased malondialdehyde content by increasing the levels of actual photochemical efficiency (), electron transport rate (ETR), and photochemical quenching () to improve the cold tolerance ability. This study provides a reference for the selection and breeding of cold-tolerant genetic resources of and the mechanism of cold-tolerance, as well as the analysis of the mechanism of adaptation of in different climatic regions of China.
L.是中国南方最重要的针叶树种之一，是森林生态系统和木材生产的支柱，但低温限制了其生长和地理分布。本研究使用来自不同耐寒性家系的30日龄幼苗，在人工气候箱中研究了不同低温胁迫下针叶和茎的形态特征、叶绿素荧光特性、保护酶以及淀粉和木质素的变化。结果表明，在低温胁迫下，该幼苗表现出表型形态和组织结构的变化。保护酶、渗透调节物质、淀粉和木质素等生理生化指标对低温胁迫有响应。耐寒家系通过提高实际光化学效率（）、电子传递速率（ETR）和光化学猝灭（）水平，增加可溶性糖、淀粉粒和木质素含量以及过氧化物酶活性，降低丙二醛含量，从而提高耐寒能力。本研究为L.耐寒遗传资源的选择和育种、耐寒机制以及中国不同气候区域L.的适应机制分析提供了参考。
