Aldholmi Mohammed, Althomali Ebtihal, Aljishi Fatema, Ahmad Rizwan, Alqathama Aljawharah, Alaswad Deema
Department of Natural Products, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi Arabia.
Plants (Basel). 2024 Aug 12;13(16):2234. doi: 10.3390/plants13162234.
The plant is traditionally used for medicinal and food purposes. However, there are limited studies on the bioactivity and cytotoxicity of its seed butter and aril oil. This study aimed to assess the antidiabetic activity and cytotoxicity of seed butter and aril oil, obtained via two different extraction methods, and compare their lipid profiles. The plant samples were collected from the Faifa mountains and extracted using a Soxhlet apparatus for hot extraction and a magnetic stirrer for cold maceration. The antidiabetic activity and cytotoxicity were evaluated using the α-amylase and MTT assays, respectively. The fatty acids were quantified utilizing gas chromatography-mass spectrometry. This study proves the impact of the extraction method on the yield, cytotoxicity, antidiabetic activity and lipid profile. The highest cytotoxicity was observed with the seed butter obtained via Soxhlet extraction. The α-amylase inhibition was observed at the highest levels with the seed butter and aril oil obtained via cold maceration. The palmitic acid (PA) and oleic acid (OA) were detected at their maximal concentrations in the seed butter obtained via Soxhlet extraction and aril oil obtained via cold maceration, respectively. This study represents an essential basis for understanding the importance of as a valuable tree for food, cosmetic and medicinal purposes. Further experiments can lead to the development of green extraction techniques and isolation of the cytotoxic and antidiabetic molecules that can be developed into new pharmaceutical products or serve as lead molecules for new drugs.
这种植物传统上用于药用和食用目的。然而，关于其种子黄油和假种皮油的生物活性和细胞毒性的研究有限。本研究旨在评估通过两种不同提取方法获得的种子黄油和假种皮油的抗糖尿病活性和细胞毒性，并比较它们的脂质谱。植物样本采自法伊法山，使用索氏提取器进行热提取，使用磁力搅拌器进行冷浸提取。分别使用α-淀粉酶和MTT试验评估抗糖尿病活性和细胞毒性。利用气相色谱-质谱法定量脂肪酸。本研究证明了提取方法对产量、细胞毒性、抗糖尿病活性和脂质谱的影响。通过索氏提取获得的种子黄油观察到最高的细胞毒性。通过冷浸获得的种子黄油和假种皮油观察到最高水平的α-淀粉酶抑制作用。棕榈酸（PA）和油酸（OA）分别在通过索氏提取获得的种子黄油和通过冷浸获得的假种皮油中检测到最大浓度。本研究为理解作为一种用于食品、化妆品和药用目的的珍贵树木的重要性提供了重要依据。进一步的实验可以导致绿色提取技术的发展以及细胞毒性和抗糖尿病分子的分离，这些分子可以开发成新的药品或作为新药的先导分子。
