麻疹病毒溶血素对丙酮的敏感性及通过吸收制备单特异性人抗溶血素
The sensitivity of measles virus haemolysin to acetone and the preparation of mono-specific human anti-haemolysin by absorption.
作者信息
Armstrong M A, Fraser K B, Shirodaria P V
出版信息
J Gen Virol. 1979 Aug;44(2):541-4. doi: 10.1099/0022-1317-44-2-541.
PMID:392051
Abstract
The haemolysin of measles virus, either in the virion or in infected cells, is functionally and antigenically sensitive to acetone. Absorption of human sera with acetone-treated, measles virus-infected cells removes antibodies to all measles virus structural antigens except haemolysin. The antibody titres of absorbed sera give good correlation in HLI, neutralization and fluorescent antibody staining on unfixed infected cells.
摘要
麻疹病毒的溶血素，无论是在病毒粒子中还是在受感染细胞中，在功能和抗原性上对丙酮敏感。用丙酮处理过的、感染麻疹病毒的细胞吸附人血清，可去除针对除溶血素之外所有麻疹病毒结构抗原的抗体。吸收后血清的抗体滴度在人白细胞抑制试验、中和试验以及对未固定感染细胞的荧光抗体染色中具有良好的相关性。
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