National Reference Laboratory (NRL) for Swine Fever, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", 06126 Perugia, Italy.
National Reference Center for Whole Genome Sequencing of Microbial Pathogens, Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", 64100 Teramo, Italy.
Viruses. 2024 Jul 24;16(8):1185. doi: 10.3390/v16081185.
The first report of African swine fever virus (ASFV) genotype II in Italy in 2022 marked the beginning of a significant invasion in at least eight Italian regions with different infection clusters. In this study, we used the multi-gene approach to investigate the epidemiological associations between ASFV strains causing cases and outbreaks in wild boar and pigs in Italy from January 2022 to the end of 2023. Our results confirm that all the tested ASFV-positive Italian samples belonged to genotype II and show high homology with genotype II ASFV sequences previously collected in Eurasian countries. Molecular characterization revealed the presence of four genetic groups in Italy. The majority of African swine fever (ASF) samples analyzed in the current study (72%) belonged to genetic group 3, which was the most representative in Europe. The results also provide evidence of the prevalence of genetic group 19 (15.9%). In addition, we identified new putative genetic groups, genetic group 25 (9.1%) and genetic group 26 (3.0%), which have never been described before. This is the first detailed report on the molecular characterization of more than 130 ASFV strains circulating in Italy.
2022 年意大利首次报告非洲猪瘟病毒（ASFV）基因型 II，标志着该病毒至少在意大利的八个不同地区的不同感染群中开始了大规模入侵。在这项研究中，我们使用多基因方法，调查了 2022 年 1 月至 2023 年底期间在意大利野猪和猪中引起病例和疫情的 ASFV 毒株的流行病学关联。我们的研究结果证实，所有检测呈阳性的意大利 ASFV 样本均属于基因型 II，与之前在欧亚国家收集的基因型 II ASFV 序列具有高度同源性。分子特征表明意大利存在四个遗传群。本研究分析的大多数非洲猪瘟（ASF）样本（72%）属于遗传群 3，这是欧洲最具代表性的遗传群。研究结果还表明，遗传群 19（15.9%）也很普遍。此外，我们还发现了新的可能遗传群，遗传群 25（9.1%）和遗传群 26（3.0%），这些遗传群以前从未被描述过。这是首次详细报告在意大利流行的 130 多种 ASFV 毒株的分子特征。
