Instituto Nacional de Investigación en Salud Pública "Leopoldo Izquieta Pérez", Dirección Técnica de Investigación, Desarrollo e Innovación, Guayaquil 090150, Ecuador.
Facultad Ciencias de la Vida, Escuela Superior Politécnica del Litoral, ESPOL, Campus Gustavo Galindo Km. 30.5 Vía Perimetral, Guayaquil 090902, Ecuador.
Viruses. 2024 Aug 2;16(8):1242. doi: 10.3390/v16081242.
The emergence and re-emergence of arthropod-borne viruses is a public health threat. For routine surveillance in public health laboratories, cost-effective and reproducible methods are essential. In this review, we address the technical considerations of high-throughput sequencing methods (HTS) for arbovirus surveillance in national health laboratories, focusing on pre-sequencing, sequencing, and post-sequencing approaches, underlining the importance of robust wet and dry laboratory workflows for reproducible analysis. We aim to provide insights for researchers and clinicians interested in arbovirus, diagnosis, and surveillance by discussing current advances in sequencing methods and bioinformatics pipelines applied to arboviruses.
虫媒病毒的出现和再现是公共卫生的威胁。在公共卫生实验室进行常规监测时，需要经济高效且可重复的方法。在这篇综述中，我们讨论了高通量测序方法（HTS）在国家卫生实验室中进行虫媒病毒监测的技术考虑因素，重点介绍了测序前、测序中和测序后的方法，强调了稳健的湿实验室和干实验室工作流程对于可重复分析的重要性。我们旨在通过讨论应用于虫媒病毒的测序方法和生物信息学管道的当前进展，为对虫媒病毒诊断和监测感兴趣的研究人员和临床医生提供见解。
