MRC-University of Glasgow-Centre for Virus Research, Garscube Campus, University of Glasgow, Glasgow G61 1QH, UK.
Viruses. 2024 Aug 21;16(8):1336. doi: 10.3390/v16081336.
symbionts introduced into mosquitoes provide a highly effective dengue virus transmission control strategy, increasingly utilised in many countries in an attempt to reduce disease burden. Whilst highly effective against dengue and other positive-sense RNA viruses, it remains unclear how effective is against negative-sense RNA viruses. Therefore, the effect of on Bunyamwera virus (BUNV) infection in was investigated using Mel and AlbB, two strains currently used in releases for dengue control, as well as Au, a strain that typically persists at a high density and is an extremely efficient blocker of positive-sense viruses. was found to reduce BUNV infection in vitro but not in vivo. Instead, BUNV caused significant impacts on density of all three strains following infection of mosquitoes. The ability of to successfully persist within the mosquito and block virus transmission is partially dependent on its intracellular density. However, reduction in density was not observed in offspring of infected mothers. This could be due in part to a lack of transovarial transmission of BUNV observed. The results highlight the importance of understanding the complex interactions between multiple arboviruses, mosquitoes and in natural environments, the impact this can have on maintaining protection against diseases, and the necessity for monitoring prevalence at release sites.
共生菌引入蚊子中提供了一种非常有效的登革热病毒传播控制策略，越来越多的国家在试图减少疾病负担时使用这种策略。虽然它对登革热和其他正链 RNA 病毒非常有效，但对于负链 RNA 病毒的效果如何仍不清楚。因此，研究了 对布尼亚病毒（BUNV）感染的影响，使用了两种目前用于登革热控制释放的菌株 Mel 和 AlbB，以及 Au 菌株，它通常以高密度持续存在，并且是正链病毒的非常有效的阻断剂。结果发现， 能够减少体外的 BUNV 感染，但不能减少体内的 BUNV 感染。相反，BUNV 感染会导致三种 菌株的密度显著降低。 能够成功在蚊子体内持续存在并阻断病毒传播的能力部分取决于其细胞内密度。然而，在感染母亲的后代中未观察到 密度降低。这可能部分是由于缺乏观察到的 BUNV 的经卵传播。研究结果强调了在自然环境中理解多种虫媒病毒、蚊子和 之间复杂相互作用的重要性，以及这对维持疾病保护的影响，以及在释放地点监测 的必要性。
