TNFSF14/LIGHT 和 CD160 在胃癌和消化性溃疡消化不良中的临床意义。

Clinical Significance of TNFSF14/LIGHT and CD160 in Gastric Cancer and Peptic Ulcer Dyspepsia.

机构信息

Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

出版信息

Asian Pac J Cancer Prev. 2024 Aug 1;25(8):2669-2677. doi: 10.31557/APJCP.2024.25.8.2669.

DOI:10.31557/APJCP.2024.25.8.2669

PMID:39205564

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11495460/

Abstract

BACKGROUND

Previous studies have reported the role of the Herpes Virus Entry Mediator (HVEM) in various cancer including gastric cancer. However, the expression level and clinical significance of CD160 and Tumor Necrosis Factor Ligand Superfamily Member 14 (TNFSF14) pathways in gastric cancer and gastric dyspepsia patients have remained unexplored.

METHODS

The study involved the collection of gastric tissue biopsies from 42 patients with non-ulcerative dyspepsia (NUD) as the control group, 43 gastric cancer (GC) patients, and 48 patients with peptic-ulcerative dyspepsia (PUD). All the patients were endoscopically examined at Imam Khomeini Hospital in Sari, Mazandaran, Iran. The expression levels of TNFSF14 and CD160 mRNA were assessed using quantitative real-time PCR (qPCR) with the SYBR Green method. Statistical analysis was performed to investigate the potential association between the clinical and experimental data.

RESULTS

Among the 133 gastric endoscopic biopsies examined, LIGHT exhibited a significant overexpression in GC patients (p-value < 0.01). Moreover, the expression of TNFSF14 was higher in GC patients with stages I and II (p-value<0.05). Furthermore, GC patients with TNM stages III+IV were accompanied by high expression levels of LIGHT (p-value < 0.01) as well as CD160 (p-value<0.05). The expression of CD160 was also higher in younger adults with PUD (p-value<0.05). Whereas TNFSF14 exhibited higher expression in older adults with GC (p-value<0.05). Furthermore, this research provided insights into the potential biological pathways and significant gene enrichment of TNFSF14 and CD160, suggesting the potential role of CD160 and TNFSF14 in the regulation of immune system in GC and PUD.

CONCLUSION

These findings suggest the possible role of LIGHT and CD160 expression in gastric cancer patients in immune dysregulation toward gastric cancer. Targeted immunotherapy that harnessing co-stimulatory molecules like LIGHT and CD160 could be a promising approach in the treatment of GC as well as potential GC tumor markers.

摘要

背景

先前的研究报告了 Herpes Virus Entry Mediator（HVEM）在包括胃癌在内的各种癌症中的作用。然而，CD160 和 Tumor Necrosis Factor Ligand Superfamily Member 14（TNFSF14）途径在胃癌和消化不良患者中的表达水平和临床意义仍未得到探索。

方法

本研究收集了 42 例非溃疡性消化不良（NUD）患者（对照组）、43 例胃癌（GC）患者和 48 例消化性溃疡性消化不良（PUD）患者的胃组织活检。所有患者均在伊朗马赞达兰省萨里的伊玛目霍梅尼医院进行内镜检查。采用 SYBR Green 法通过实时定量 PCR（qPCR）评估 TNFSF14 和 CD160 mRNA 的表达水平。进行统计学分析以研究临床和实验数据之间的潜在关联。

结果

在检查的 133 例胃内镜活检中，GC 患者中 LIGHT 表现出显著的过表达（p 值<0.01）。此外，TNFSF14 在 GC 患者的 I 期和 II 期表达较高（p 值<0.05）。此外，GC 患者的 TNM 分期 III+IV 伴有 LIGHT（p 值<0.01）和 CD160（p 值<0.05）的高表达水平。年轻的 PUD 患者的 CD160 表达也更高（p 值<0.05）。而 GC 中年龄较大的患者 TNFSF14 表达较高（p 值<0.05）。此外，本研究深入探讨了 TNFSF14 和 CD160 的潜在生物学途径和显著基因富集，提示 CD160 和 TNFSF14 可能在 GC 和 PUD 中的免疫系统调节中发挥作用。