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红海海绵提取物 Callyspongia siphonella 及其代谢产物在结肠癌细胞 2D 和 3D 培养物中的抗癌活性。

Red Sea Sponge Extract Callyspongia siphonella and its Metabolites Induced Anticancer Activity in 2D and 3D Culture of Colon Cancer Cells.

机构信息

Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

Regenerative Medicine Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

出版信息

Asian Pac J Cancer Prev. 2024 Aug 1;25(8):2869-2876. doi: 10.31557/APJCP.2024.25.8.2869.

DOI:10.31557/APJCP.2024.25.8.2869
PMID:39205585
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11495439/
Abstract

Colorectal Cancer (CRC) significantly contributes to global cancer-related mortality and morbidity. Callyspongia siphonella (Callyspongia sp.), a Red Sea sponge, has shown promising activity as an anticancer extract and a source of anticancer-active compounds. This study sought to determine the effects of Callyspongia siphonella and its metabolites on HCT-116 colon cancer cells. Cell viability assays showed that Callyspongia sp. inhibited in a dose-dependent manner, the growth of HCT-116 cell lines with IC50 values of 64.8±17 ug/ml on 2D culture and 141.1±6.8 ug/ml on 3D culture. The purified compounds Sipholenol-A and Sipholenone-A have an IC50 of 48.9±2.2 uM and 47.1±1.2 uM respectively. Following Callyspongia sp. treatment of HCT-116, cell cycle analysis showed arrest at G2/M.flow cytometry analysis showed an increase in total apoptosis due to Callyspongia sp treatment. Moreover, mitochondria membrane potential has been reported to be depolarized due to Callyspongia sp. which is an extra sign of apoptosis. Further investigations are needed to explain the particular underlying mechanisms of Callyspongia sp. extract and its metabolites Sipholenol-A and Sipholenone-A to explore their therapeutic potential in treating colon cancer.

摘要

结直肠癌（CRC）是导致全球癌症相关死亡率和发病率的主要原因之一。红海海绵 Callyspongia siphonella（Callyspongia sp.）具有良好的抗癌活性，是一种抗癌提取物和抗癌活性化合物的来源。本研究旨在确定 Callyspongia siphonella 及其代谢产物对 HCT-116 结肠癌细胞的影响。细胞活力测定表明，Callyspongia sp.以剂量依赖的方式抑制 HCT-116 细胞系的生长，在二维培养中的 IC50 值为 64.8±17 ug/ml，在三维培养中的 IC50 值为 141.1±6.8 ug/ml。纯化的化合物 Sipholenol-A 和 Sipholenone-A 的 IC50 值分别为 48.9±2.2 uM 和 47.1±1.2 uM。在 Callyspongia sp.处理 HCT-116 后，细胞周期分析显示 G2/M 期阻滞。流式细胞术分析显示由于 Callyspongia sp.处理导致总凋亡增加。此外，由于 Callyspongia sp.导致线粒体膜电位去极化，这是凋亡的另一个迹象。需要进一步的研究来解释 Callyspongia sp.提取物及其代谢物 Sipholenol-A 和 Sipholenone-A 的特定潜在机制，以探索它们在治疗结肠癌方面的治疗潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbe0/11495439/2a8d645161e7/APJCP-25-2869-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbe0/11495439/8806911cdf94/APJCP-25-2869-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbe0/11495439/3b53048be5e3/APJCP-25-2869-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbe0/11495439/2a8d645161e7/APJCP-25-2869-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbe0/11495439/8806911cdf94/APJCP-25-2869-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbe0/11495439/5048acfd2187/APJCP-25-2869-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbe0/11495439/5b52d6d1584d/APJCP-25-2869-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbe0/11495439/8fff88a40bfc/APJCP-25-2869-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbe0/11495439/3b53048be5e3/APJCP-25-2869-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbe0/11495439/2a8d645161e7/APJCP-25-2869-g006.jpg

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本文引用的文献

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Chemical and biological diversity of new natural products from marine sponges: a review (2009-2018).海洋海绵新天然产物的化学与生物多样性综述（2009 - 2018年）
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Screening and Molecular Docking of Bioactive Metabolites of the Red Sea Sponge as Potential Antimicrobial Agents.
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