Cui Xiangran, Jiao Jianhang, Yang Lili, Wang Yang, Jiang Weibo, Yu Tong, Li Mufeng, Zhang Han, Chao Bo, Wang Zhonghan, Wu Minfei
Department of Orthopedics, The Second Hospital of Jilin University Changchun, 130041, PR China.
Orthopaedic Research Institute of Jilin Province, Changchun, 130041, PR China.
Mater Today Bio. 2024 Aug 8;28:101198. doi: 10.1016/j.mtbio.2024.101198. eCollection 2024 Oct.
Bioprinting is a groundbreaking technology that enables precise distribution of cell-containing bioinks to construct organoid models that accurately reflect the characteristics of tumors . By incorporating different types of tumor cells into the bioink, the heterogeneity of tumors can be replicated, enabling studies to simulate real-life situations closely. Precise reproduction of the arrangement and interactions of tumor cells using bioprinting methods provides a more realistic representation of the tumor microenvironment. By mimicking the complexity of the tumor microenvironment, the growth patterns and diffusion of tumors can be demonstrated. This approach can also be used to evaluate the response of tumors to drugs, including drug permeability and cytotoxicity, and other characteristics. Therefore, organoid models can provide a more accurate oncology research and treatment simulation platform. This review summarizes the latest advancements in bioprinting to construct tumor organoid models. First, we describe the bioink used for tumor organoid model construction, followed by an introduction to various bioprinting methods for tumor model formation. Subsequently, we provide an overview of existing bioprinted tumor organoid models.
生物打印是一项开创性技术,它能够精确分配含有细胞的生物墨水,以构建能够准确反映肿瘤特征的类器官模型。通过将不同类型的肿瘤细胞纳入生物墨水中,可以复制肿瘤的异质性,使研究能够更紧密地模拟现实情况。使用生物打印方法精确再现肿瘤细胞的排列和相互作用,能更真实地呈现肿瘤微环境。通过模拟肿瘤微环境的复杂性,可以展示肿瘤的生长模式和扩散情况。这种方法还可用于评估肿瘤对药物的反应,包括药物渗透性和细胞毒性等其他特性。因此,类器官模型能够提供一个更精确的肿瘤学研究和治疗模拟平台。本综述总结了生物打印构建肿瘤类器官模型的最新进展。首先,我们描述用于构建肿瘤类器官模型的生物墨水,接着介绍形成肿瘤模型的各种生物打印方法。随后,我们概述现有的生物打印肿瘤类器官模型。