Song Shi-Jian, Diao Hai-Ping, Guo Yong-Feng, Hwang Inhwan
Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China.
Beijing Life Science Academy (BLSA), Beijing, China.
Biodes Res. 2024 Aug 28;6:0047. doi: 10.34133/bdr.0047. eCollection 2024.
Plants and their use as bioreactors for the generation of recombinant proteins have become one of the hottest topics in the field of Plant Biotechnology and Plant Synthetic Biology. Plant bioreactors offer superior engineering potential compared to other types, particularly in the realm of subcellular accumulation strategies for increasing production yield and quality. This review explores established and emerging strategies for subcellular accumulation of recombinant proteins in tobacco bioreactors, highlighting recent advancements in the field. Additionally, the review provides reference to the crucial initial step of selecting an optimal subcellular localization for the target protein, a design that substantially impacts production outcomes.
植物及其作为生产重组蛋白的生物反应器的应用已成为植物生物技术和植物合成生物学领域最热门的话题之一。与其他类型的生物反应器相比，植物生物反应器具有卓越的工程潜力，尤其是在提高产量和质量的亚细胞积累策略方面。本综述探讨了烟草生物反应器中亚细胞积累重组蛋白的既定策略和新兴策略，突出了该领域的最新进展。此外，本综述还提到了为目标蛋白选择最佳亚细胞定位这一关键初始步骤，这一设计对生产结果有重大影响。
