Chen Tao, Tan Yi, Song Ying, Wei Guangwu, Li Zhiqiang, Wang Ximing, Yang Jing, Millman Alexander J, Chen Minmei, Liu Deping, Huang Tao, Jiao Ming, He Weitao, Zhao Xiuchang, Greene Carolyn M, Kile James C, Zhou Suizan, Zhang Ran, Zeng Xiaoxu, Guo Qian, Wang Dayan
Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Guangxi Autonomous Regional Center for Disease Control and Prevention, Nanning 530028, China.
Biosaf Health. 2023;5(1):30-36. doi: 10.1016/j.bsheal.2022.12.006.
We conducted environmental surveillance to detect avian influenza viruses circulating at live poultry markets (LPMs) and poultry farms in Guangxi Autonomous Region, China, where near the China-Vietnam border. From November through April 2017-2018 and 2018-2019, we collected environmental samples from 14 LPMs, 4 poultry farms, and 5 households with backyard poultry in two counties of Guangxi and tested for avian influenza A, H5, H7, and H9 by real-time reverse transcription-polymerase chain reaction (rRT-PCR). In addition, we conducted four cross-sectional questionnaire surveys among stall owners on biosecurity practices in LPMs of two study sites. Among 16,713 environmental specimens collected and tested, the median weekly positive rate for avian influenza A was 53.6% (range = 33.5% - 66.0%), including 25.2% for H9, 4.9% for H5, and 21.2% for other avian influenza viruses A subtypes, whereas a total of two H7 positive samples were detected. Among the 189 LPM stalls investigated, most stall owners (73.0%) sold chickens and ducks. Therefore, continued surveillance of the avian influenza virus is necessary for detecting and responding to emerging trends in avian influenza virus epidemiology.
我们在中国广西壮族自治区靠近中越边境的活禽市场和家禽养殖场开展了环境监测，以检测正在传播的禽流感病毒。在2017年11月至2018年4月以及2018年11月至2019年4月期间，我们从广西两个县的14个活禽市场、4个家禽养殖场和5个有后院家禽的家庭采集了环境样本，并通过实时逆转录聚合酶链反应（rRT-PCR）检测甲型禽流感病毒、H5、H7和H9。此外，我们对两个研究地点活禽市场的摊主进行了四次关于生物安全措施的横断面问卷调查。在收集并检测的16713份环境样本中，甲型禽流感病毒的每周阳性率中位数为53.6%（范围=33.5%-66.0%），其中H9为25.2%，H5为4.9%，其他甲型禽流感病毒亚型为21.2%，而共检测到两份H7阳性样本。在调查的189个活禽市场摊位中，大多数摊主（73.0%）出售鸡和鸭。因此，持续监测禽流感病毒对于发现和应对禽流感病毒流行病学的新趋势很有必要。
