Yuan Shuai, Du Ming, Li Xianhui, Xu Ke, Zhang Kaining, Liu Xiaoya, Wang Jiangxin
School of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.
Hainan Chenhai Aquatic Co., Ltd., Sanya City, Hainan, China.
Front Nutr. 2024 Aug 14;11:1460675. doi: 10.3389/fnut.2024.1460675. eCollection 2024.
The microalga Chlorella sp. NeZha, recently isolated from a balcony environment, shows significant adaptability across various salinity conditions, including seawater (SeaW), freshwater (FreshW), and high salinity levels (45‰). This study investigates its potential for sustainable aquaculture and biotechnological applications.
Morphological and genetic identification were conducted using optical microscopy and DNA sequencing. The microalga was cultivated in a 400 L outdoor photobioreactor, and its biochemical composition, including chlorophyll a, carbohydrate, protein, and lipid content, was analyzed. Its compatibility with zooplankton and growth in aquaculture wastewater were also evaluated.
Chlorella sp. NeZha produced chlorophyll a at concentrations exceeding seaweed and Spirulina by 10- and 5-fold, respectively, with a dry weight chlorophyll a content of 34.25 mg/g and 25 pg./cell. The microalga also contained carbohydrate (33%), protein (20%), and lipids (~14%). It was compatible with zooplankton species, such as rotifers and brine shrimp, and showed promising growth in aquaculture wastewater.
The findings suggest that Chlorella sp. NeZha is a viable candidate for sustainable aquaculture and biotechnological applications, offering high nutritional value and environmental resilience. Its adaptability to diverse salinity conditions and ability to thrive in wastewater highlight its potential for bioremediation and use as feedstock for zooplankton. Further research is recommended to optimize its cultivation and explore broader applications.
微藻小球藻属哪吒藻，最近从阳台环境中分离得到，在各种盐度条件下都表现出显著的适应性，包括海水（SeaW）、淡水（FreshW）和高盐度水平（45‰）。本研究调查了其在可持续水产养殖和生物技术应用方面的潜力。
使用光学显微镜和DNA测序进行形态学和遗传学鉴定。该微藻在400升室外光生物反应器中培养，并分析其生化组成，包括叶绿素a、碳水化合物、蛋白质和脂质含量。还评估了其与浮游动物的相容性以及在水产养殖废水中的生长情况。
小球藻属哪吒藻产生的叶绿素a浓度分别比海藻和螺旋藻高出10倍和5倍，干重叶绿素a含量为34.25毫克/克，每细胞25皮克。该微藻还含有碳水化合物（约33%）、蛋白质（约20%）和脂质（约14%）。它与轮虫和卤虫等浮游动物物种相容，并在水产养殖废水中显示出良好的生长态势。
研究结果表明，小球藻属哪吒藻是可持续水产养殖和生物技术应用的一个可行候选者，具有高营养价值和环境适应能力。它对不同盐度条件的适应性以及在废水中茁壮成长的能力突出了其在生物修复和作为浮游动物饲料原料方面的潜力。建议进一步研究以优化其培养并探索更广泛的应用。
