Leong S W, Terasaki P I
Transfusion. 1985 Mar-Apr;25(2):149-51. doi: 10.1046/j.1537-2995.1985.25285169209.x.
A simple microtest for red cell (RBC) typing was developed which required 0.002 ml of reagents per test. After mixing RBCs with antibody, the microtray was incubated upside down for 10 to 15 minutes at 37 degrees C, and 10 minutes at room temperature. The trays were read after reinversion and allowing 10 to 15 minutes for the RBCs to settle. Antibodies must be selected that react under these conditions. Two examples each of anti-A, -B, -C, -D, -E, -c, -M, and -N were tested against a panel of 500 individuals. The results were generally concordant with each other and with the conventional tube test results. The procedure is simple to perform and involves minimal costs for reagent antisera.
开发了一种用于红细胞（RBC）分型的简单微量试验，每次试验需要0.002毫升试剂。将红细胞与抗体混合后，微量托盘倒置在37摄氏度下孵育10至15分钟，在室温下孵育10分钟。再次倒置托盘并让红细胞沉降10至15分钟后读取结果。必须选择在这些条件下发生反应的抗体。针对一组500名个体测试了抗A、抗B、抗C、抗D、抗E、抗c、抗M和抗N的各两个实例。结果彼此之间以及与传统试管试验结果总体一致。该程序操作简单，试剂抗血清成本最低。
