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[检测接种脓性免疫原者血清中抗铜绿假单胞菌抗体的免疫酶分析法]

[Immunoenzyme analysis method for detecting anti-Pseudomonas aeruginosa antibodies in persons inoculated with pyoimmunogen].

作者信息

Severtsova M K, Vaĭda M B, Varró R

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1985 Jan(1):55-9.

PMID:3920846
Abstract

The possibility of using the indirect ELISA techniques for evaluating the level of the post-vaccinal production of humoral antibodies in donors immunized with Pyoimmunogen. P. aeruginosa vaccine, has been studied. The specificity and high resolution of this test system, based on the immobilization of the antigens of the vaccinal preparation on a solid-phase carrier, have been demonstrated. A rational method for the evaluation of specific antibody titers with due regard to the spectrophotometric data indicating the results of the reaction and the degree of the dilution of the serum under test has been proposed.

摘要

研究了使用间接酶联免疫吸附测定(ELISA)技术评估接种绿脓杆菌免疫原(铜绿假单胞菌疫苗)的供体中体液抗体疫苗接种后产生水平的可能性。已经证明了该检测系统的特异性和高分辨率,该系统基于将疫苗制剂的抗原固定在固相载体上。提出了一种合理的方法来评估特异性抗体滴度,同时适当考虑指示反应结果的分光光度数据和被测血清的稀释度。

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