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[在临床实践中使用多价红细胞诊断剂测定铜绿假单胞菌抗体]

[Use of a polyvalent erythrocyte diagnostic agent for determining antibodies to Pseudomonas aeruginosa in clinical practice].

作者信息

Aleksandrova I A, Titova T I, Aleksandrov A D, Moroz A F

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1989 Jan(1):46-50.

PMID:2496541
Abstract

The use of polyvalent erythrocyte diagnosticum prepared on the basis of 5 polysaccharide antigens of P. aeruginosa slime, isolated from strains belonging to the most widespread serovars, makes it possible to check up the humoral response of donors after their immunization with P. aeruginosa polyvalent corpuscular vaccine with the aim of obtaining anti-P. aeruginosa donor plasma. Antibody titers, determined in the passive hemagglutination test with the use of the proposed diagnosticum and corresponding to a serum dilution of 1:320 and greater, age tentatively diagnostic, which may be indicative of P. aeruginosa in the development of purulent septic complications in patients. The use of the passive hemagglutination test with the newly developed polyvalent erythrocyte diagnosticum makes it possible to check up the specific response of patients having P. aeruginosa infection in the process of their treatment with anti-P. aeruginosa hyperimmune plasma used as a part of complex therapy.

摘要

使用基于从属于最广泛血清型的菌株中分离出的5种铜绿假单胞菌黏液多糖抗原制备的多价红细胞诊断制剂,能够在供体用铜绿假单胞菌多价微粒疫苗免疫后检查其体液反应,目的是获得抗铜绿假单胞菌供体血浆。在使用所提议的诊断制剂进行的被动血凝试验中测定的抗体效价,相当于血清稀释度为1:320及更高时,初步具有诊断意义,这可能表明患者发生化脓性败血症并发症时存在铜绿假单胞菌。使用新开发的多价红细胞诊断制剂进行被动血凝试验,能够在使用抗铜绿假单胞菌超免疫血浆作为综合治疗一部分对铜绿假单胞菌感染患者进行治疗的过程中检查其特异性反应。

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