School of Nutrition and Public Health, College of Health, Oregon State University, Corvallis, OR, United States; Moore Family Center, College of Health, Oregon State University, Corvallis, OR, United States.
Department of Nutritional Sciences, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada; Department of Pediatrics, Sinai Health, Toronto, Ontario, Canada; Rogers Hixon Ontario Human Milk Bank, Sinai Health, Toronto, Ontario, Canada; Translational Medicine Program, The Hospital for Sick Children, Toronto, Ontario, Canada.
Food Chem. 2025 Jan 1;462:140973. doi: 10.1016/j.foodchem.2024.140973. Epub 2024 Aug 23.
High-pressure processing (HPP) of donor human milk (DM) minimally impacts the concentration and bioactivity of some important bioactive proteins including lactoferrin, and bile salt-stimulated lipase (BSSL) compared to Holder pasteurization (HoP), yet the impact of HPP and subsequent digestion on the full array of proteins detectable by proteomics remains unclear. We investigated how HPP impacts undigested proteins in DM post-processing and across digestion by proteomic analysis. Each pool of milk (n = 3) remained raw, or was treated by HPP (500 MPa, 10 min) or HoP (62.5 °C, 30 min), and underwent dynamic in vitro digestion simulating the preterm infant. In the meal, major proteins were minimally changed post-processing. HPP-treated milk proteins better resisted proximal digestion (except for immunoglobulins, jejunum 180 min) and the extent of undigested proteins after gastric digestion of major proteins in HPP-treated milk was more similar to raw (e.g., BSSL, lactoferrin, macrophage-receptor-1, CD14, complement-c3/c4, xanthine dehydrogenase) than HoP.
高压处理（HPP）对捐赠人乳（DM）中一些重要生物活性蛋白的浓度和生物活性的影响较小，与 Holder 巴氏消毒（HoP）相比，包括乳铁蛋白和胆汁盐刺激的脂肪酶（BSSL），但 HPP 及其随后的消化对蛋白质组学可检测的全套蛋白质的影响仍不清楚。我们通过蛋白质组学分析研究了 HPP 如何影响加工后 DM 中的未消化蛋白以及整个消化过程。每批牛奶（n=3）保持原状，或经 HPP（500 MPa，10 min）或 HoP（62.5°C，30 min）处理，并进行模拟早产儿的动态体外消化。在餐中，主要蛋白质在加工后几乎没有变化。与 HoP 相比，经 HPP 处理的牛奶蛋白在近端消化过程中更好地抵抗消化（除免疫球蛋白外，空肠 180 分钟），并且在胃消化后，主要蛋白质的未消化蛋白的程度更接近原始状态（例如，BSSL、乳铁蛋白、巨噬细胞受体-1、CD14、补体 C3/C4、黄嘌呤脱氢酶）。
