Univ. Lille, Inserm, CHU Lille, U1286 - INFINITE - Institute for Translational Research in Inflammation, F-59000, Lille, France.
Division of Gastroenterology, Hepatology and Nutrition, Department of Paediatrics, Jeanne de Flandre Children's Hospital, CHU Lille, F-59000, Lille, France.
Pediatr Res. 2024 Jun;95(7):1749-1753. doi: 10.1038/s41390-024-03022-9. Epub 2024 Jan 27.
The microbiological safety of donor milk (DM) is commonly ensured by Holder pasteurization (HoP, 62.5 °C for 30 min) in human milk banks despite its detrimental effects on bioactive factors. We compared the antimicrobial properties of DM after Holder pasteurization treatment or High Hydrostatic Pressure processing (HHP, 350 MPa at 38 °C), a non-thermal substitute for DM sterilization.
We assessed lactoferrin and lysozyme concentrations in raw, HHP- and HoP-treated pools of DM (n = 8). The impact of both treatments was evaluated on the growth of Escherichia coli and Group B Streptococcus in comparison with control media (n = 4). We also addressed the effect of storage of HHP treated DM over a 6-month period (n = 15).
HHP milk demonstrated similar concentrations of lactoferrin compared with raw milk, while it was significantly decreased by HoP. Lysozyme concentrations remained stable regardless of the condition. Although a bacteriostatic effect was observed against Escherichia coli at early timepoints, a sharp bactericidal effect was observed against Group B Streptococcus. Unlike HoP, these results were significant for HHP compared to controls. Stored DM was well and safely preserved by HHP.
Our study demonstrates that this alternative sterilization method shows promise for use with DM in human milk banks.
Antimicrobial activity of donor milk after High Hydrostatic Pressure treatment has not been clearly evaluated. Donor milk lactoferrin is better preserved by High Hydrostatic Pressure than conventional Holder pasteurization, while lysozyme concentration is not affected by either treatment. As with Holder pasteurization, High Hydrostatic Pressure preserves donor milk bacteriostatic activity against E. coli in addition to bactericidal activity against Group B Streptococcus. Donor milk treated by High Hydrostatic Pressure can be stored safely for 6 months.
尽管 Holder 巴氏消毒法（62.5°C 下 30 分钟）对生物活性因子有不利影响，但在人乳库中，仍常通过该法确保捐赠奶（DM）的微生物安全性。我们比较了 DM 经 Holder 巴氏消毒法处理或高压处理（HHP，38°C 下 350MPa）后的抗菌特性，后者是非 DM 灭菌的替代热法。
我们评估了原始、HHP 和 HoP 处理的 DM 池（n=8）中的乳铁蛋白和溶菌酶浓度。将两种处理方法的影响与对照培养基（n=4）进行比较，评估其对大肠杆菌和 B 组链球菌生长的影响。我们还研究了在 6 个月期间储存 HHP 处理的 DM 的效果（n=15）。
HHP 奶的乳铁蛋白浓度与原始奶相似，而 HoP 则显著降低。溶菌酶浓度保持稳定，与条件无关。尽管在早期观察到对大肠杆菌的抑菌作用，但对 B 组链球菌观察到急剧的杀菌作用。与 HoP 不同，与对照组相比，这些结果对 HHP 有统计学意义。与 HoP 不同，HHP 可安全地储存 DM。
我们的研究表明，这种替代灭菌方法有望在人乳库中用于 DM。
HHP 处理后 DM 的抗菌活性尚未得到明确评估。与传统的 Holder 巴氏消毒法相比，HHP 更能保留 DM 中的乳铁蛋白，而溶菌酶浓度不受两种处理方法的影响。与 Holder 巴氏消毒法一样，HHP 保留了 DM 对大肠杆菌的抑菌活性，以及对 B 组链球菌的杀菌活性。经 HHP 处理的 DM 可安全储存 6 个月。
