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The fluorometric determination of europium ion concentration as used in time-resolved fluoroimmunoassay.

作者信息

Dakubu S, Ekins R P

出版信息

Anal Biochem. 1985 Jan;144(1):20-6. doi: 10.1016/0003-2697(85)90078-8.

DOI:10.1016/0003-2697(85)90078-8
PMID:3920928
Abstract

A model of the relationships between optimal fluorescence yield, Eu3+ ion concentration, and the concentration of beta-diketone in the determination of Eu3+ ion concentration in aqueous solutions as used in time-resolved fluoroimmunoassays was developed. The model, in addition to optimizing the determination of the metal ion, also presents a new simple and rapid method for the determination of stability constants for the formation of beta-diketone-Eu3+ complexes. It is shown how the method may be extended to determine the formation constants for Eu3+ with other chelates.

摘要

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