Keelan J A, France J T, Barling P M
Department of Obstetrics and Gynaecology, National Women's Hospital, Auckland, New Zealand.
Clin Chem. 1987 Dec;33(12):2292-5.
Time-resolved fluoroimmunoassays that make use of lanthanide chelates as labels require the addition of an enhancement solution to elicit the formation of a fluorescent lanthanide complex. All solutions previously described are based on 2-naphthoyltrifluoroacetone (NTA), a beta-diketone. Currently, this compound is not commercially available. We report here the properties and performance of an enhancement solution prepared with a commercially available beta-diketone, thenoyltrifluoroacetone. Use of this solution in a commercial time-resolved fluoroimmunoassay gave results essentially identical to those obtained with the NTA-based solution, although fluorescence emission was approximately 27% lower. The lower fluorescence yield did not, however, significantly reduce assay sensitivity. We conclude that this solution represents a viable and highly economical alternative to the preparation currently in use, particularly for laboratories wishing to develop their own assays based on lanthanide fluorescence.
利用镧系螯合物作为标记的时间分辨荧光免疫测定法需要添加增强溶液以引发荧光镧系络合物的形成。先前描述的所有溶液均基于β-二酮2-萘甲酰三氟丙酮（NTA）。目前，该化合物没有商业供应。我们在此报告用市售β-二酮噻吩甲酰三氟丙酮制备的增强溶液的性质和性能。在商业时间分辨荧光免疫测定中使用该溶液得到的结果与用基于NTA的溶液获得的结果基本相同，尽管荧光发射约低27％。然而，较低的荧光产率并未显著降低测定灵敏度。我们得出结论，该溶液是目前使用的制剂的可行且高度经济的替代品，特别是对于希望开发基于镧系元素荧光的自身测定法的实验室。
