Animal and Bioscience Research Department, Teagasc, Animal & Grassland Research and Innovation Centre, Grange, Dunsany, Co. Meath, Ireland.
Livestock Systems Research Department, Teagasc, Animal & Grassland Research and Innovation Centre, Grange, Dunsany, Co. Meath, Ireland.
Sci Rep. 2024 Aug 29;14(1):20046. doi: 10.1038/s41598-024-70669-z.
Identifying and breeding cattle that are more feed efficient is of great benefit to beef production. Additionally, it is crucial that genes contributing to feed efficiency are robust across varying management settings including dietary source as well as being relevant across contrasting breeds of cattle. The aim of this study was to determine miRNAs that are contributing to the expression of residual feed intake (RFI) across two breeds and dietary sources. miRNA profiling was undertaken in Longissimus dorsi tissue of Charolais and Holstein-Friesian steers divergent for RFI phenotype following two contrasting consecutive diets (high-forage and high-concentrate). Ten miRNA were identified as differentially expressed (adj. P < 0.1) across the breed and diet contrasts examined. Of particular interest was the differential expression of miR-2419-5p and miR-2415-3p, both of which were up-regulated in the Low-RFI Charolais steers across each dietary phase. Pathway analysis of target mRNA genes of differentially expressed miRNA revealed enrichment (P < 0.05) for pathways including metabolic related pathways, insulin receptor signalling, adipogenesis as well as pathways related to skeletal muscle growth. These results provide insight into the skeletal muscle miRNAome of beef cattle and their potential molecular regulatory mechanisms relating to feed efficiency in beef cattle.
鉴定和培育更具饲料效率的牛对牛肉生产有很大的好处。此外，具有饲料效率贡献的基因在不同的管理环境中具有稳健性非常重要，包括饮食来源，并且在不同的牛品种中具有相关性。本研究的目的是确定在两个品种和两种饮食来源中与剩余饲料摄入量（RFI）表达有关的 miRNA。对 RFI 表型不同的夏洛莱牛和荷斯坦-弗里生牛的背最长肌组织进行了 miRNA 谱分析，这些牛在两种截然不同的连续饮食（高草料和高浓缩物）下存在差异。在研究的品种和饮食对比中，有 10 个 miRNA 被确定为差异表达（adj. P < 0.1）。特别值得关注的是 miR-2419-5p 和 miR-2415-3p 的差异表达，它们在低 RFI 夏洛莱牛中在每个饮食阶段都被上调。差异表达 miRNA 的靶 mRNA 基因的通路分析显示，代谢相关通路、胰岛素受体信号通路、脂肪生成以及与骨骼肌生长相关的通路都有富集（P < 0.05）。这些结果为牛肉牛的骨骼肌 miRNA 组及其与牛肉牛饲料效率相关的潜在分子调节机制提供了深入了解。
