Uchida Yuki, Nagashima Kei, Yuri Kazunari
Department of Neurobiology and Anatomy, Kochi Medical School, Kochi University, Kochi, Japan.
Laboratory of Integrative Physiology (Body Temperature and Fluid Laboratory), Faculty of Human Sciences, Waseda University, Saitama, Japan; Institute of Applied Brain Sciences, Waseda University, Saitama, Japan.
Brain Res. 2017 Sep 1;1670:125-134. doi: 10.1016/j.brainres.2017.06.009. Epub 2017 Jun 9.
Rats place their tails underneath their bodies in the cold (tail-hiding behavior), which is a behavioral indicator of thermoregulation. The aim of the present study was to elucidate the effect of estradiol (E) on tail-hiding behavior and neural activity assessed by immunohistochemistry. Ovariectomized rats were implanted with a silastic tube with or without E underneath the dorsal skin (E(-) and E(+) groups), and exposed to 27°C, 16°C, and 10°C for 2h with continuous body temperature (T), tail skin temperature (T), and behavioral measurements. cFos immunoreactive (cFos-IR) cells in the insula, secondary somatosensory cortex, medial preoptic nucleus, parastrial nucleus, amygdala, and lateral parabrachial nucleus were counted. T and T were not different between the E(-) and E(+) groups. At 16°C, the duration and the onset of tail-hiding behavior in the E(+) group were greater than that in the E(-) group. The number of cFos-IR cells in the insula of the E(-) group was greater than that of the E(+) group in rats kept at 16°C. E might modulate tail-hiding behavior of female rats at 16°C, and the insula may be involved in the response.
在寒冷环境中，大鼠会将尾巴藏于身体下方（藏尾行为），这是一种体温调节的行为指标。本研究的目的是阐明雌二醇（E）对藏尾行为以及通过免疫组织化学评估的神经活动的影响。对去卵巢大鼠在背部皮肤下植入含或不含E的硅橡胶管（E(-)组和E(+)组），并在27°C、16°C和10°C环境下暴露2小时，同时持续测量体温（T）、尾皮肤温度（T）以及行为表现。对岛叶、次级体感皮层、视前内侧核、旁室核、杏仁核和外侧臂旁核中的cFos免疫反应性（cFos-IR）细胞进行计数。E(-)组和E(+)组之间的T和T没有差异。在16°C时，E(+)组的藏尾行为持续时间和开始时间均长于E(-)组。在16°C环境下饲养的大鼠中，E(-)组岛叶中cFos-IR细胞的数量多于E(+)组。E可能在16°C时调节雌性大鼠的藏尾行为，且岛叶可能参与了这一反应。
