Physical Education Department, Wuhan University, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei province, People's Republic of China.
School of Physical Education and Equestrian, Wuhan Business University, No. 816 Dongfeng Avenue, Wuhan Economic and Technological Development Zone, Hubei Province, People's Republic of China.
Anal Methods. 2024 Sep 19;16(36):6079-6097. doi: 10.1039/d4ay01002g.
Nonenzymatic electrochemical biosensors, renowned for their high sensitivity, multi-target analysis capabilities, and miniaturized integration, align well with the requirements of non-invasive, multi-index integrated, continuous monitoring, and user-friendly wearable biosensors in sports science. In the past three years, novel strategies targeting specific responses to sports biomarkers have opened new avenues for applications in sports science. However, these advancements also pose challenges in achieving adequate sensitivity and specificity for online analysis of complex sweat bio-samples. Our article focuses on three key nonenzymatic electrochemical biosensing strategies: antigen-antibody reactions, nucleic acid aptamer recognition, and molecular imprinting capture. We delve into strategies to enhance specificity and sensitivity in the application of biosensors in sports science, including shortening signal transduction paths through built-in signal probes, increasing reaction sites through increased surface area and the introduction of nanostructures, multi-target analyses, and microfluidic techniques.
非酶电化学生物传感器以其高灵敏度、多目标分析能力和小型化集成而闻名，非常适合运动科学领域对非侵入性、多指标集成、连续监测和用户友好型可穿戴生物传感器的需求。在过去的三年中，针对运动生物标志物的特定反应的新型策略为运动科学中的应用开辟了新的途径。然而，这些进展也在实现对复杂汗液生物样本的在线分析的足够灵敏度和特异性方面提出了挑战。我们的文章重点介绍了三种关键的非酶电化学生物传感策略：抗原-抗体反应、核酸适体识别和分子印迹捕获。我们深入探讨了在运动科学中应用生物传感器时提高特异性和灵敏度的策略，包括通过内置信号探针缩短信号转导路径、通过增加表面积和引入纳米结构增加反应位点、多目标分析和微流控技术。
