正常和甲状腺功能减退的新生儿及儿童干血斑中人类红细胞碳酸酐酶I的浓度。

Human erythrocyte carbonic anhydrase I concentrations in dried blood spots from normal and hypothyroid neonates and children.

作者信息

Shepherd J N, Spencer N, Hulse J A

出版信息

Clin Biochem. 1985 Feb;18(1):62-6. doi: 10.1016/s0009-9120(85)80025-4.

Abstract

The ELISA technique was used to assay carbonic anhydrase I (HCAI) in hemolysates prepared by the elution of dried blood samples from Guthrie cards. The ratio HCAI (mg)/hemoglobin (g) measured in blood samples eluted from Guthrie cards was not significantly different from that determined in aliquots of the same blood samples after storage as erythrocyte (RBC) lysates at -20 degrees C, provided that the dried blood was eluted within three weeks of collection. The normal neonatal mean (SD) RBC HCAI concentrations were 2.05 (1.01) and 1.82 (0.86) mg HCAI/g hemoglobin for females and males respectively. Erythrocyte HCAI concentrations gradually rose with age, approaching normal adult levels by 16 years. Blood from hypothyroid neonates and hypothyroid infants on treatment gave normal HCAI/hemoglobin ratios.

摘要

采用酶联免疫吸附测定（ELISA）技术，对从格思里卡片上洗脱干血样制备的溶血产物中的碳酸酐酶I（HCAI）进行检测。从格思里卡片上洗脱的血样中测得的HCAI（毫克）/血红蛋白（克）比值，与相同血样在-20℃下作为红细胞（RBC）裂解物储存后的等分试样中测得的比值相比，无显著差异，前提是干血样在采集后三周内洗脱。正常新生儿红细胞HCAI浓度的均值（标准差），女性为2.05（1.01）毫克HCAI/克血红蛋白，男性为1.82（0.86）毫克HCAI/克血红蛋白。红细胞HCAI浓度随年龄逐渐升高，到16岁时接近正常成人水平。甲状腺功能减退新生儿和接受治疗的甲状腺功能减退婴儿的血液中，HCAI/血红蛋白比值正常。