Shepherd J N, Spencer N, Bidwell D E, Voller A
Clin Biochem. 1982 Oct;15(5):248-51. doi: 10.1016/s0009-9120(82)97019-9.
A method is described for the assay of human erythrocyte (RBC) carbonic anhydrase I (HCAI) by the enzyme linked immunosorbent assay. The method was found to be a simple, reliable and precise technique and gave a mean C.V. for 40 samples, assayed in quadruplicate, of 3.32% and a range of 0.84-5.9. The mean erythrocyte HCAI value and standard deviation for 20 normal men and women were respectively 16.9 +/- 3.4 and 15.4 +/- 2.1 mg HCAI/g haemoglobin. The use of heparin as an anticoagulant interfered with the assay resulting in apparent HCAI concentrations as low as 60% of those obtained using EDTA.
描述了一种通过酶联免疫吸附测定法检测人红细胞（RBC）碳酸酐酶I（HCAI）的方法。该方法被发现是一种简单、可靠且精确的技术，对40个样本进行四次重复测定，平均变异系数为3.32%，范围为0.84 - 5.9。20名正常男性和女性的红细胞HCAI平均值和标准差分别为16.9 +/- 3.4和15.4 +/- 2.1毫克HCAI/克血红蛋白。使用肝素作为抗凝剂会干扰测定，导致表观HCAI浓度低至使用乙二胺四乙酸（EDTA）时所获浓度的60%。
