Aliakbar S, Brown P R
Molecular Biology and Biophysics Section, Division of Biomedical Sciences, King's College London, Strand, UK.
Clin Biochem. 1996 Apr;29(2):157-64. doi: 10.1016/0009-9120(95)02021-7.
The aim of the present study was to determine the erythrocyte CAI and CAII concentrations in fetal blood over a wide gestational range, and compare levels to those found in neonates and adults.
Human erythrocyte CAI and CAII concentrations were measured in fetal (n=38), neonatal (n=10) and adult (n=30) blood, using enzyme-linked immunosorbent assays (ELISA). For the measurement of CAII, a new ELISA method was developed.
The ELISA method was found to be simple, sensitive, economical, and precise. The normal mean levels of erythrocyte CAI and CAII in adults with standard deviation were 13.68 +/- 2.79, and 1.59 +/- 0.21 mg/g Hb, respectively. The corresponding values in cord blood obtained at delivery at 38-40 weeks gestation were 1.20 +/- 0.68 and 0.46 +/- 0.13 mg/g Hb. The mean CAI/CAII ratio in adults was 8.8 and, in newborns, it was 2.5. The normal mean fetal erythrocyte CAI concentration increased significantly with gestation from 39 microgram/g Hb at 20 weeks to 380 microgram/g Hb at 38 weeks of gestation. Similarly, the CAII concentration increased from 53 microgram/g Hb at 20 weeks to 120 microgram/g Hb at 38 weeks of gestation. The CAI to CAII ratio also increased with gestation from 0.9 at 20 weeks to 2.5 at 38 weeks.
本研究旨在测定不同孕周胎儿血液中红细胞碳酸酐酶I（CAI）和碳酸酐酶II（CAII）的浓度，并与新生儿和成人的水平进行比较。
采用酶联免疫吸附测定法（ELISA）测量胎儿（n = 38）、新生儿（n = 10）和成人（n = 30）血液中的人红细胞CAI和CAII浓度。对于CAII的测量，开发了一种新的ELISA方法。
发现ELISA方法简单、灵敏、经济且精确。成人红细胞CAI和CAII的正常平均水平及标准差分别为13.68±2.79和1.59±0.21mg/g血红蛋白。妊娠38 - 40周分娩时脐血中的相应值分别为1.20±0.68和0.46±0.13mg/g血红蛋白。成人的平均CAI/CAII比值为8.8，新生儿为2.5。正常胎儿红细胞CAI浓度随孕周显著增加，从妊娠20周时的39微克/克血红蛋白增加到妊娠38周时的380微克/克血红蛋白。同样，CAII浓度从妊娠20周时的53微克/克血红蛋白增加到妊娠38周时的120微克/克血红蛋白。CAI与CAII的比值也随孕周从妊娠20周时的0.9增加到妊娠38周时的2.5。
