Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Clermont-Ferrand, France.
Division of Prosthodontics, Faculty of Dentistry, Thammasat University, Khlong Luang, Thailand.
J Biomed Mater Res B Appl Biomater. 2024 Sep;112(9):e35477. doi: 10.1002/jbm.b.35477.
Our laboratory recently developed a new class of high surface area, honeycomb Nanoclay Microsphere Framework absorbents (NMFs) that prompt rapid hemostasis. In the present work, we propose a novel approach to develop antibacterial Topical Hemostatic Agents (THAs) by anchoring silver nanoparticles (AgNPs) onto NMFs. This combination was obtained by a chemical co-reduction approach, followed by freeze-processing, and was shown to ensure stability and on-site delivery of AgNPs, without altering the hemostatic properties of NMFs. Silver-loaded NMFs showed no change in their unique architecture and led to a 55% increase in clot strength, compared to standard control plasma or commercially available THA, and a significant decrease in mean fibrin fiber diameter. Silver nanoparticles were successfully released when solubilized and prevented the growth of both Pseudomonas aeruginosa and Staphylococcus aureus at concentrations of 22 and 30 ppm of silver released, respectively. Overall, cell mortality was between 9.1 ± 5.1% and 6.3 ± 3.2%, depending on AgNP concentration, confirming a low cytotoxicity. Silver-loaded nanoclay microsphere frameworks appear to constitute promising candidates as topical hemostatic agents for secondary management of hemostasis when infection control is needed.
我们实验室最近开发了一种新型高比表面积、蜂窝纳米粘土微球骨架吸收剂（NMFs），能迅速止血。在本工作中，我们提出了一种通过将纳米银颗粒（AgNPs）锚定在 NMFs 上来制备抗菌局部止血剂（THAs）的新方法。该复合物通过化学共还原法获得，然后进行冷冻处理，确保了 AgNPs 的稳定性和现场输送，而不会改变 NMFs 的止血性能。载银 NMFs 其独特的结构没有发生变化，与标准对照血浆或市售 THA 相比，其凝血块强度增加了 55%，平均纤维蛋白纤维直径显著减小。当银被溶解时，银纳米颗粒成功释放，并在分别释放 22 和 30 ppm 银的浓度下，能够阻止铜绿假单胞菌和金黄色葡萄球菌的生长。总的来说，细胞死亡率在 9.1±5.1%和 6.3±3.2%之间，这取决于 AgNP 的浓度，证实了其低细胞毒性。载银纳米粘土微球骨架似乎是一种很有前途的局部止血剂候选材料，可用于需要控制感染时的二级止血管理。
