Yu Zihao, Shi Yuan, Lin Jialiang, Wang Yan, He Chunlu, Cheng Jianhua
Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, College of Environment and Energy, South China University of Technology, Guangzhou 510006, China; South China Institute of Collaborative Innovation, Dongguan 523808, China.
Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, College of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
Int J Biol Macromol. 2025 Aug;319(Pt 1):145130. doi: 10.1016/j.ijbiomac.2025.145130. Epub 2025 Jun 16.
Overusing antibiotics has accelerated the rapid emergence and spread of resistant strains, posing a serious threat to global public health. To address this challenge, this study developed a synergistic antibacterial composite material, CAT@ZIF-8/AgNPs, based on the metal-organic framework material ZIF-8, catalase (CAT), and silver nanoparticles (AgNPs). First, CAT was encapsulated in ZIF-8, effectively enhancing the enzyme's stability and catalytic activity in complex environments. AgNPs were then grown in situ on the surface of CAT@ZIF-8, which endowed the composite material with broad-spectrum antibacterial properties and synergistically enhanced the antibacterial effect through silver ion release and enzymatic catalysis. Enzyme activity tests demonstrated that CAT@ZIF-8 exhibited excellent stability, retaining over 50 % activity at pH 11, above 80 % at 50 °C, and over 70 % in organic solvents, significantly outperforming free CAT under all tested conditions. Antibacterial tests indicated that the composite material exhibited significant synergistic antibacterial effects against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Furthermore, cytotoxicity tests revealed that the composite material demonstrated good biocompatibility with human keratinocytes (HaCaT) at concentrations up to 500 μg/mL. The CAT@ZIF-8/AgNPs composite material showcases efficient antibacterial performance and good biosafety through the synergistic action of enzymes and silver nanoparticles, indicating broad application prospects.
过度使用抗生素加速了耐药菌株的迅速出现和传播，对全球公共卫生构成严重威胁。为应对这一挑战，本研究基于金属有机框架材料ZIF-8、过氧化氢酶（CAT）和银纳米颗粒（AgNPs）开发了一种协同抗菌复合材料CAT@ZIF-8/AgNPs。首先，将CAT封装在ZIF-8中，有效提高了酶在复杂环境中的稳定性和催化活性。然后，AgNPs在CAT@ZIF-8表面原位生长，赋予复合材料广谱抗菌性能，并通过银离子释放和酶催化协同增强抗菌效果。酶活性测试表明，CAT@ZIF-8表现出优异的稳定性，在pH值为11时保留超过50%的活性，在50℃时超过80%，在有机溶剂中超过70%，在所有测试条件下均显著优于游离CAT。抗菌测试表明，该复合材料对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌具有显著的协同抗菌作用。此外，细胞毒性测试表明，该复合材料在浓度高达500μg/mL时与人角质形成细胞（HaCaT）具有良好的生物相容性。CAT@ZIF-8/AgNPs复合材料通过酶和银纳米颗粒的协同作用展现出高效的抗菌性能和良好的生物安全性，具有广阔的应用前景。
