Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
Environ Pollut. 2024 Nov 15;361:124848. doi: 10.1016/j.envpol.2024.124848. Epub 2024 Aug 29.
Profenofos (PFF) is a commonly used organophosphorus insecticide that requires strict monitoring due to its potential environmental, ecological, and human health risks originating from residues in soil and water systems, as well as accumulation in crops. In this study, a novel monoclonal antibody (mAb) specific to PFF was prepared for the first time and the recognition mechanism was investigated through molecular simulation. Subsequently, a mAb-based colloidal gold immunochromatographic assay (GICA) was developed for the rapid screening of PFF in fruit and vegetable samples. The mAb exhibited an IC value of 12.9 ng/mL, and limit of detection (LOD) of 4.6 ng/mL, respectively in indirect competitive immunosorbent enzyme-linked immunosorbent assay (ic-ELISA). After optimization, the developed GICA exhibited a visual limit of detection (vLOD) of 20 ng/mL and a quantitative of detection (qLOD) of 5.2 ng/mL, with a linear range from 10.0 to 83.8 ng/mL. Good correlation was observed between the results of GICA and standard Gas Chromatography-Tandem Mass Spectrometry (GC-MS/MS) in matrix and recovery test. The developed GICA can be used for rapid sample detection within 15 min, which is an excellent tool for screening PFF in foods and environmental samples.
丙溴磷（PFF）是一种常用的有机磷杀虫剂，由于其在土壤和水系中的残留以及在作物中的积累所带来的潜在环境、生态和人类健康风险，需要进行严格监测。本研究首次制备了针对 PFF 的新型单克隆抗体（mAb），并通过分子模拟研究了其识别机制。随后，建立了基于 mAb 的胶体金免疫层析法（GICA），用于快速筛选水果和蔬菜样品中的 PFF。该 mAb 在间接竞争酶联免疫吸附试验（ic-ELISA）中的 IC 为 12.9ng/mL，LOD 为 4.6ng/mL。经过优化，开发的 GICA 的可视检测限（vLOD）为 20ng/mL，定量检测限（qLOD）为 5.2ng/mL，线性范围为 10.0 至 83.8ng/mL。在基质和回收试验中，GICA 与标准气相色谱-串联质谱（GC-MS/MS）的结果具有良好的相关性。该开发的 GICA 可用于 15 分钟内的快速样品检测，是筛选食品和环境样品中 PFF 的理想工具。
