Liwicki R, Paterson A, MacDonald M J, Broda P
J Bacteriol. 1985 May;162(2):641-4. doi: 10.1128/jb.162.2.641-644.1985.
This paper reports the isolation of phenoloxidase-negative mutants of the white-rot fungus Phanerochaete chrysosporium and the results of a survey of idiophasic functions among these mutants. The mutant strains were isolated from a medium containing o-anisidine after gamma irradiation of wild-type spores and fell into four classes, divided by the manner in which they mineralized 14C-lignin wheat lignocellulose. Examples are strain LMT7, which degraded lignin at a rate similar to that of the wild type; strain LMT26, in which degradation was enhanced; strain LMT16, whose degradation rate was apparently unaffected, although the onset of lignin attack was delayed compared with that in the wild type; and strain LMT24, which was unable to evolve significant amounts of 14CO2 from the radiolabeled substrate. The mutants were not necessarily defective in other functions associated with idiophasic activities (intracellular cyclic AMP levels, sporulation, extracellular glucan production, veratryl alcohol synthesis). We conclude that phenoloxidase activity as detected by the o-anisidine plate test is not necessary for lignin degradation. In addition, mutations resulting in the loss of lignin-degrading ability were not necessarily pleiotropic with other idiophasic functions.
本文报道了白腐真菌黄孢原毛平革菌酚氧化酶阴性突变体的分离以及对这些突变体中特定阶段功能的调查结果。突变菌株是在对野生型孢子进行γ射线照射后，从含有邻茴香胺的培养基中分离得到的，根据它们矿化14C-木质素小麦木质纤维素的方式可分为四类。例如，LMT7菌株降解木质素的速率与野生型相似；LMT26菌株中降解作用增强；LMT16菌株的降解速率显然未受影响，尽管与野生型相比，木质素攻击的起始时间有所延迟；LMT24菌株无法从放射性标记底物中释放出大量的14CO2。这些突变体在与特定阶段活性相关的其他功能（细胞内环状AMP水平、孢子形成、细胞外葡聚糖产生、藜芦醇合成）方面不一定存在缺陷。我们得出结论，通过邻茴香胺平板试验检测到的酚氧化酶活性对于木质素降解并非必需。此外，导致木质素降解能力丧失的突变不一定与其他特定阶段功能呈多效性。
