Bartlett W A
Department of Biochemical Medicine, Ninewells Hospital, Dundee U.K.
J Chromatogr. 1989 Aug 25;493(1):1-14. doi: 10.1016/s0378-4347(00)82703-6.
The effects of pH, ionic strength, organic modifier, heptanesulphonic acid and citric acid content of a high-performance liquid chromatography mobile phase on the chromatographic and electrochemical behaviour of norepinephrine, epinephrine, dopamine, 3,4-dihydroxybenzylamine, 3,4-dihydroxyphenylethylene glycol, 3,4-dihydroxyphenylalanine and 3,4-dihydroxyphenylacetic acid in a reversed-phase system have been systematically studied. Optimal mobile phase conditions have been derived allowing the separation and reductive-mode detection of these compounds, applicable to both alumina and ion-paired solvent extracts of plasma. It is demonstrated that mobile phase composition significantly affects the sensitivity of a triple-electrode electrochemical detection system, in reductive and oxidative modes, and that electrochemical pre-treatment of mobile phase is required to attain maximum detector sensitivity in the reductive mode.
系统研究了高效液相色谱流动相的pH值、离子强度、有机改性剂、庚烷磺酸和柠檬酸含量对去甲肾上腺素、肾上腺素、多巴胺、3,4-二羟基苄胺、3,4-二羟基苯乙二醇、3,4-二羟基苯丙氨酸和3,4-二羟基苯乙酸在反相系统中的色谱和电化学行为的影响。得出了最佳流动相条件，可实现这些化合物的分离和还原模式检测，适用于血浆的氧化铝和离子对溶剂提取物。结果表明，流动相组成显著影响三电极电化学检测系统在还原和氧化模式下的灵敏度，并且需要对流动相进行电化学预处理以在还原模式下获得最大的检测器灵敏度。
