Mitchell D, Ibrahim S, Gusterson B A
J Histochem Cytochem. 1985 May;33(5):491-5. doi: 10.1177/33.5.3921605.
Detailed comparative studies have been carried out using a wide range of aqueous and nonaqueous based fixatives in order to select the optimal fixation schedule for the immunohistochemical localization of four antigens: type IV collagen, actin, Factor VIII, and epithelial membrane antigen (EMA). Modified methacarn fixation provides an ideal combination of maximum staining and morphological preservation for these antigens. Enhanced immunoreactivity with this fixative was also noted for a broad spectrum of antigens, including myoglobin, glial fiber acidic protein, keratin, myosin, laminin, prostatic acid phosphatase, alpha-fetoprotein, and carcinoembryonic antigen. Although it is not recommended for most cell surface antigens, this fixative may have a definite place in diagnostic surgical pathology.
为了选择针对四种抗原（IV型胶原、肌动蛋白、凝血因子VIII和上皮膜抗原（EMA））进行免疫组织化学定位的最佳固定方案，已经使用了多种水性和非水性固定剂进行了详细的比较研究。改良的甲醇-氯仿固定法为这些抗原提供了最大染色和形态保存的理想组合。对于包括肌红蛋白、胶质纤维酸性蛋白、角蛋白、肌球蛋白、层粘连蛋白、前列腺酸性磷酸酶、甲胎蛋白和癌胚抗原在内的广泛抗原，也观察到使用这种固定剂后免疫反应性增强。尽管不推荐用于大多数细胞表面抗原，但这种固定剂在诊断手术病理学中可能有一定的地位。
