Markham R B, Pier G B, Goellner J J, Mizel S B
J Immunol. 1985 Jun;134(6):4112-7.
T lymphocytes from immune mice can adoptively transfer protection against infection with the extra-cellular Gram-negative bacterium Pseudomonas aeruginosa to nonimmune recipients, and in vitro, immune T cells are able to kill these bacteria. Earlier studies indicated that this killing is mediated by a bactericidal lymphokine. Those studies also showed that macrophages enhance this in vitro T cell killing but do not directly participate in the bacterial killing, nor do macrophages function to present antigen to T cells. The current studies demonstrate that the ability of macrophages to enhance T cell killing can be replaced by macrophage culture supernatants or by purified recombinant interleukin 1 (IL 1). In addition, the macrophage supernatant-induced enhancement can also be blocked by antibody to purified IL 1. These studies also demonstrate that the T cell subset that serves as the final effector cell in the killing process is the Lyt-1-, 2,3+, I-J+ phenotype.
来自免疫小鼠的T淋巴细胞能够将针对细胞外革兰氏阴性菌铜绿假单胞菌感染的保护作用过继转移给非免疫受体，并且在体外，免疫T细胞能够杀死这些细菌。早期研究表明，这种杀伤作用是由一种杀菌性淋巴因子介导的。那些研究还表明，巨噬细胞可增强这种体外T细胞杀伤作用，但不直接参与细菌杀伤，巨噬细胞也不具有向T细胞呈递抗原的功能。目前的研究表明，巨噬细胞增强T细胞杀伤的能力可被巨噬细胞培养上清液或纯化的重组白细胞介素1（IL-1）所取代。此外，针对纯化IL-1的抗体也可阻断巨噬细胞上清液诱导的增强作用。这些研究还表明，在杀伤过程中作为最终效应细胞的T细胞亚群是Lyt-1-、2,3+、I-J+表型。
