Prokesová L, Dung D H, Trebichavský I, Formánková E, Stĕpánková V, John C
1st Medical Faculty, Charles University, Prague, Czech Republic.
Folia Microbiol (Praha). 1994;39(5):428-34. doi: 10.1007/BF02814451.
Human mononuclear leukocytes kill Staphylococcus aureus cells in vitro. The killing of the bacteria takes place even in the absence of antibodies. The presence of antibodies (in an autologous inactivated serum) usually enhances the antibacterial activity of mononuclear leukocytes. In some cases, however, this activity is markedly decreased by the serum, probably depending of the spectrum of antibodies contained in the serum. The antibacterial activity of mononuclear leukocytes is mostly due to monocytes because their depletion causes substantial drop or the activity disappearance. We failed to demonstrate in the case of S. aureus the antibacterial cytotoxicity of T lymphocytes described by some authors dealing with Gram-negative bacteria. Large differences in the structure of the bacterial cell wall underlie apparently the different sensitivity of G+ and G- bacteria to some protective mechanisms of the host. In the antibacterial assay against S. aureus, electron microscopy revealed a maximal activation of monocytes which phagocytized the bacteria although extracellular killing is not excluded. Electronoptical findings point also to a possible participation of NK cells in the antibacterial cytotoxicity against S. aureus.
人单核白细胞在体外可杀死金黄色葡萄球菌细胞。即使在没有抗体的情况下，细菌的杀灭也会发生。抗体（在自体灭活血清中）的存在通常会增强单核白细胞的抗菌活性。然而，在某些情况下，血清会显著降低这种活性，这可能取决于血清中所含抗体的种类。单核白细胞的抗菌活性主要归因于单核细胞，因为去除单核细胞会导致活性大幅下降或消失。在金黄色葡萄球菌的情况下，我们未能证明一些研究革兰氏阴性菌的作者所描述的T淋巴细胞的抗菌细胞毒性。细菌细胞壁结构的巨大差异显然是革兰氏阳性菌和革兰氏阴性菌对宿主某些保护机制敏感性不同的基础。在针对金黄色葡萄球菌的抗菌试验中，电子显微镜显示单核细胞被最大程度地激活，它们吞噬了细菌，不过细胞外杀灭也不能排除。电子光学研究结果还表明，自然杀伤细胞可能参与了对金黄色葡萄球菌的抗菌细胞毒性作用。
