Key Laboratory of TCM-information Engineer of State Administration of TCM, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China.
Key Laboratory of TCM-information Engineer of State Administration of TCM, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China.
Phytomedicine. 2024 Nov;134:155992. doi: 10.1016/j.phymed.2024.155992. Epub 2024 Aug 26.
Polygoni Cuspidati Rhizoma et Radix (Huzhang in Chinese), refers to the root and rhizome of Polygonum cuspidatum Sieb. et Zucc. Huzhang is commonly used in clinical practice for the prevention and treatment of diabetes and its complications, but its active components and regulatory mechanisms have not yet been thoroughly analyzed.
The network pharmacology combined with multi-omics analysis will be employed to dissect the substance basis and action mechanism of Huzhang in exerting its anti-diabetic activity.
This study employed phenotypic indicators for baseline assessment, followed by integrated analysis using network pharmacology, metabolomics, transcriptomics, and qPCR technology to elucidate the active components and pharmacological mechanisms of Huzhang.
The analysis of network pharmacology revealed that polydatin is a potential active component responsible for the anti-T2DM pharmacological effects of Huzhang. In vivo experimental results demonstrated that polydatin significantly regulates blood glucose, lipid levels, liver function, and liver pathological damage in diabetic rats. Analysis results from transcriptomics, metabolomics, and qPCR validation showed that polydatin comprehensively regulates glucose and lipid metabolism in T2DM by modulating bile acid metabolism, fatty acid oxidation, and lipogenesis.
Polydatin is a key component of Huzhang in treating T2DM, and its regulatory mechanisms are diverse, indicating significant development potential.
虎杖来源于蓼科植物虎杖Polygonum cuspidatum Sieb. et Zucc. 的干燥根茎和根。虎杖在临床上常用于防治糖尿病及其并发症，但对其活性成分和作用机制尚缺乏深入分析。
采用网络药理学结合多组学分析方法，解析虎杖发挥抗糖尿病作用的物质基础和作用机制。
本研究采用表型指标进行基线评估，然后通过网络药理学、代谢组学、转录组学和 qPCR 技术的综合分析，阐明虎杖的活性成分和药理机制。
网络药理学分析表明，白藜芦醇苷是虎杖发挥抗 2 型糖尿病作用的潜在活性成分。体内实验结果表明，白藜芦醇苷能显著调节糖尿病大鼠的血糖、血脂水平、肝功能和肝病理损伤。转录组学、代谢组学和 qPCR 验证结果表明，白藜芦醇苷通过调节胆汁酸代谢、脂肪酸氧化和脂肪生成，全面调节 2 型糖尿病的糖脂代谢。
白藜芦醇苷是虎杖治疗 2 型糖尿病的关键成分，其作用机制多样，具有显著的开发潜力。
