溃疡棒状杆菌菌株ATCC 9015培养滤液中缺乏白喉毒素的证据。

Proof for the lack of diphtheria toxin in culture filtrates of Corynebacterium ulcerans strain ATCC 9015.

作者信息

Souckova A, Soucek A

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1981;249(4):520-6.

Abstract

Extensive efforts to identify the diphtheria toxin in culture filtrates of C. ulcerans strain ATCC 9015 was unsuccessful. The culture filtrates did contain a component (called D-antigen) which formed a precipitin line with commercial diphtheria antitoxin. This component was purified by immunosorbent techniques using immobilized preparations of diphtheria antitoxin. It was shown, that D-antigen has a molecular weight of 84000, is not toxic for guinea-pigs, and has nothing in common with purified diphtheria toxin when tested in gel-diffusion tests. The lack of diphtheria toxin in form of its fragments in culture filtrates concentrated 20-fold was proved by the highly sensitive and specific viroimmunoassay.

摘要

为鉴定溃疡棒状杆菌菌株ATCC 9015培养滤液中的白喉毒素，人们付出了巨大努力，但均未成功。培养滤液中确实含有一种成分（称为D抗原），它能与市售白喉抗毒素形成沉淀线。该成分通过使用固定化白喉抗毒素制剂的免疫吸附技术进行纯化。结果表明，D抗原的分子量为84000，对豚鼠无毒，在凝胶扩散试验中与纯化的白喉毒素没有共同之处。通过高度灵敏和特异的病毒免疫测定法证明，浓缩20倍的培养滤液中不存在白喉毒素片段形式的白喉毒素。