Makioka A, Kobayashi A
J Protozool. 1985 Feb;32(1):153-6. doi: 10.1111/j.1550-7408.1985.tb03030.x.
Experiments were carried out to determine what subcellular fractions of Tetrahymena pyriformis could, after inoculation into mice, activate macrophages to kill Toxoplasma gondii in vitro. Peritoneal macrophages from mice inoculated intraperitoneally with cilia, pellicles, mitochondria, and microsomes exhibited strong toxoplasmacidal activity and had an enhanced capacity to release hydrogen peroxide (H2O2) by stimulation of a membrane-active agent as compared with resident macrophages. In contrast, macrophages from mice inoculated with macronuclei and postmicrosomal supernatant showed no toxoplasmacidal activity and a low level of H2O2 release. Similar dose response was observed on the active subcellular fractions with regard to the degree of macrophage activation. Treatment of the active subcellular fractions with heating and trypsin markedly reduced their activity.
进行了实验以确定梨形四膜虫的哪些亚细胞组分在接种到小鼠体内后，能够激活巨噬细胞在体外杀死刚地弓形虫。经腹腔接种纤毛、表膜、线粒体和微粒体的小鼠的腹腔巨噬细胞表现出很强的抗弓形虫活性，并且与驻留巨噬细胞相比，通过膜活性剂刺激释放过氧化氢（H2O2）的能力增强。相比之下，接种了大核和微粒体后上清液的小鼠的巨噬细胞没有抗弓形虫活性，且H2O2释放水平较低。在活性亚细胞组分方面，观察到了关于巨噬细胞激活程度的类似剂量反应。用加热和胰蛋白酶处理活性亚细胞组分显著降低了它们的活性。
