Kim C T, Chung P R, Im K I
Department of Parasitology, College of Medicine, Yonsei University, Seoul, Korea.
Kisaengchunghak Chapchi. 1991 Jun;29(2):129-37. doi: 10.3347/kjp.1991.29.2.129.
Tetrahymena pyriformis is a free-living ciliate protozoan in the freshwater system. Experiments were carried out to determine whether intraperitoneal administration of T. pyriformis (GL strain) to mice activates macrophages to be able to kill Toxoplasma gondii tachyzoites in vitro. Mice were also injected intraperitoneally with several synthetic activators; dimethyldioctadecylammonium bromide (DDA), dextran sulfate, complete Freund's adjuvant (CFA) as well as Toxoplasma and Tetrahymena lysates in order to activate mouse peritoneal macrophages. One week after the administration of activators, peritoneal cells were harvested and the adherent macrophages were challenged with Toxoplasma tachyzoites. Macrophage monolayers were then fixed with absolute methanol after washing, and stained with Giemsa solution. The percentage of the adherent cells infected and total number of organisms per 100 macrophages were calculated to make toxoplasmacidal activity of macrophages according to the cultivation time. Peritoneal macrophages from mice administered with Tetrahymena exhibited significant protection against target parasites as compared with those treated with synthetic activators. Among non-biological synthetic activators, DDA was evaluated as an excellent activator.
梨形四膜虫是淡水系统中一种自由生活的纤毛原生动物。进行实验以确定给小鼠腹腔注射梨形四膜虫（GL株）是否能激活巨噬细胞，使其能够在体外杀死刚地弓形虫速殖子。还给小鼠腹腔注射了几种合成激活剂；二甲基二十八烷基溴化铵（DDA）、硫酸葡聚糖、完全弗氏佐剂（CFA）以及弓形虫和四膜虫裂解物，以激活小鼠腹腔巨噬细胞。在给予激活剂一周后，收集腹腔细胞，并用刚地弓形虫速殖子攻击贴壁巨噬细胞。然后在洗涤后用无水甲醇固定巨噬细胞单层，并用吉姆萨溶液染色。根据培养时间计算每100个巨噬细胞中被感染的贴壁细胞百分比和生物体总数，以得出巨噬细胞的杀弓形虫活性。与用合成激活剂处理的小鼠相比，用四膜虫处理的小鼠的腹腔巨噬细胞对目标寄生虫表现出显著保护作用。在非生物合成激活剂中，DDA被评估为一种优秀的激活剂。
