Makioka A, Kobayashi A
Microbiol Immunol. 1984;28(7):777-85. doi: 10.1111/j.1348-0421.1984.tb00733.x.
Phagocytosis, enzyme activities and capacity to release hydrogen peroxide (H2O2) and superoxide anion (O2-) of peritoneal macrophages from mice inoculated with Tetrahymena pyriformis, a free-living ciliate, were examined in comparison with resident and BCG-activated macrophages. Fc receptor-mediated phagocytosis of sheep erythrocytes was markedly increased in Tetrahymena-activated macrophages to the same level as that seen in BCG-activated ones. Tetrahymena-activated macrophages showed an increased level of acid phosphatase (lysosomal enzyme) and a reduced level of alkaline phosphodiesterase I (plasma membrane ectoenzyme) as compared with resident macrophages. Similar changes in the activities of the two enzymes were also observed in BCG-activated macrophages. Both Tetrahymena- and BCG-activated macrophages exhibited more enhanced capacity to release H2O2 and O2- than resident macrophages when stimulated with phorbol myristate acetate. In the macrophages from mice inoculated with varying doses of Tetrahymena, a significant correlation was observed between the increased capacity of H2O2 and O2- release as observed in the present study, and the enhanced toxoplasmacidal activity as observed in a previous study, in a dose-dependent fashion.
检测了接种梨形四膜虫（一种自由生活的纤毛虫）的小鼠腹腔巨噬细胞的吞噬作用、酶活性以及释放过氧化氢（H2O2）和超氧阴离子（O2-）的能力，并与常驻巨噬细胞和卡介苗激活的巨噬细胞进行了比较。梨形四膜虫激活的巨噬细胞中，Fc受体介导的绵羊红细胞吞噬作用显著增加，达到了与卡介苗激活的巨噬细胞相同的水平。与常驻巨噬细胞相比，梨形四膜虫激活的巨噬细胞酸性磷酸酶（溶酶体酶）水平升高，碱性磷酸二酯酶I（质膜外切酶）水平降低。在卡介苗激活的巨噬细胞中也观察到了这两种酶活性的类似变化。在用佛波酯肉豆蔻酸酯刺激时，梨形四膜虫和卡介苗激活的巨噬细胞释放H2O2和O2-的能力均比常驻巨噬细胞更强。在接种不同剂量梨形四膜虫的小鼠的巨噬细胞中，本研究中观察到的H2O2和O2-释放能力的增加与先前研究中观察到的弓形虫杀灭活性增强之间存在显著的剂量依赖性相关性。
