Montgomery G W, Martin G B, Le Bars J, Pelletier J
J Reprod Fertil. 1985 Mar;73(2):457-63. doi: 10.1530/jrf.0.0730457.
Three experiments were conducted to study changes in pulsatile secretion of LH and FSH during the breeding season or anoestrus in ovariectomized Ile-de-France ewes fed different amounts of the phyto-oestrogen coumestrol. In Exp. 1, conducted during the breeding season, ewes (3-4 per group) were fed lucerne supplying 4, 18 or 30 mg coumestrol per ewe per day for 15 days. Experiments 2 and 3 were conducted during seasonal anoestrus. In Exp. 2, ewes (4 per group) were fed lucerne supplying coumestrol concentrations ranging from 4 to 38 mg/ewe/day for 15 days. In Exp. 3, ewes (10 per group) were fed lucerne supplying 14 or 125 mg coumestrol/ewe/day for 15 days. During the breeding season, an increased concentration of coumestrol in the diet significantly decreased the amplitude of LH pulses. There were no effects on LH pulse frequency or on FSH concentrations. During seasonal anoestrus, there were no significant effects on LH pulse frequency, or amplitude and no significant effect on FSH concentration. These results show that high concentrations of coumestrol in lucerne diets would not explain seasonal variation in LH pulse frequency in ovariectomized ewes. However, lucerne diets with increased coumestrol concentrations can influence LH release during the breeding season.
进行了三项实验，以研究在繁殖季节或季节性发情间期，给切除卵巢的法兰西岛母羊饲喂不同量的植物雌激素香豆雌酚时，促黄体生成素（LH）和促卵泡生成素（FSH）脉冲式分泌的变化。在实验1中，于繁殖季节进行，每组3 - 4只母羊，给它们饲喂紫花苜蓿，每只母羊每天提供4、18或30毫克香豆雌酚，持续15天。实验2和实验3在季节性发情间期进行。在实验2中，每组4只母羊，给它们饲喂香豆雌酚浓度为4至38毫克/母羊/天的紫花苜蓿，持续15天。在实验3中，每组10只母羊，给它们饲喂香豆雌酚含量为14或125毫克/母羊/天的紫花苜蓿，持续15天。在繁殖季节，日粮中香豆雌酚浓度升高显著降低了LH脉冲的幅度。对LH脉冲频率或FSH浓度没有影响。在季节性发情间期，对LH脉冲频率、幅度没有显著影响，对FSH浓度也没有显著影响。这些结果表明，紫花苜蓿日粮中高浓度的香豆雌酚并不能解释切除卵巢母羊LH脉冲频率的季节性变化。然而，香豆雌酚浓度增加的紫花苜蓿日粮会影响繁殖季节期间LH的释放。
