Nwannenna A I, Madej A, Lundh T J, Fredriksson G
Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, Uppsala.
Acta Vet Scand. 1994;35(2):173-83. doi: 10.1186/BF03548345.
The influence of phytoestrogens was studied in 3 ovariectomized Swedish Friesian heifers fed 20 kg of 100% red clover silage per heifer/day for 14 days. Behaviour, reproductive organs and pituitary response to exogenous gonadotropin-releasing hormone (GnRH) injections were monitored. Clinical effects like oedema and mucous discharge in the vulva, presence of milky fluid in the mammae and increases in teat size and the cross-sectional distance of the uterus were observed in heifers fed red clover silage. Fluid accumulation in the uterus, visualized by means of ultrasonography, had still not disappeared 30 days after the red clover silage had been completely withdrawn. Red clover silage appeared to reduce the magnitude and duration of the pituitary response to GnRH injections.
对3头去卵巢的瑞典弗里斯兰小母牛进行了植物雌激素影响的研究，每头小母牛每天饲喂20千克100%红三叶草青贮饲料，持续14天。监测了行为、生殖器官以及垂体对外源促性腺激素释放激素（GnRH）注射的反应。在饲喂红三叶草青贮饲料的小母牛中观察到了诸如外阴水肿和黏液分泌、乳房中出现乳汁样液体以及乳头大小和子宫横截面积增加等临床效应。通过超声检查可视化的子宫内积液，在完全停止饲喂红三叶草青贮饲料30天后仍未消失。红三叶草青贮饲料似乎降低了垂体对GnRH注射反应的幅度和持续时间。
